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231226s2023 xx |||||o 00| ||eng c |
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|a 10.1021/acs.langmuir.3c00362
|2 doi
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|a pubmed25n1192.xml
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|a (DE-627)NLM357851595
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|a (NLM)37283301
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|a DE-627
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|c DE-627
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|a eng
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|a Long, Hongyang
|e verfasserin
|4 aut
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|a Immobilization of Laccase by Alkali-Etched Bimetallic CoCu-MOF To Enhance Enzyme Loading and Congo Red Degradation
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|c 2023
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 21.06.2023
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|a Date Revised 26.06.2023
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a In this work, the strategy of immobilizing enzymes in bimetallic-organic frameworks was adopted to overcome the disadvantages of free laccases. The surface amino-silanizing of bimetallic CoCu-MOF-H hydrothermally synthesized was performed by (3-Aminopropyl)triethoxysilane (APTES). Then, glutaraldehyde was used as the cross-linking agent, laccase was covalently grafted onto CoCu-MOF-H-APTES to prepare Lac-CoCu-MOF-H-APTE. In addition, CoCu-MOF-OH also was synthesized by alkali etching of CoCu-MOF-H, and Lac-CoCu-MOF-OH-APTES composites were obtained by a similar strategy. The result showed that the relative enzyme activity of Lac-CoCu-MOF-OH-APTES exhibited 264.02% (1.8 times than that of Lac-CoCu-MOF-H-APTES) after six cycles of stability tests, while the free enzyme was almost inactivated. Moreover, the Congo red (CR) removal rate of Lac-CoCu-MOF-OH-APTES exceeded 95% within 1 h and exceeded 89.18% after six cycles at pH 3.5 and 50 °C. This work has the potential to provide a broader application prospect for CR degradation by laccase in the future
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Laccase
|2 NLM
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|a EC 1.10.3.2
|2 NLM
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|a Enzymes, Immobilized
|2 NLM
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|a Congo Red
|2 NLM
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|a 3U05FHG59S
|2 NLM
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|a Alkalies
|2 NLM
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|a Li, Xueping
|e verfasserin
|4 aut
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|a Liu, Xiaochen
|e verfasserin
|4 aut
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| 700 |
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|a Wang, Wanying
|e verfasserin
|4 aut
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|a Yang, Xia
|e verfasserin
|4 aut
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| 700 |
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|a Wu, Zhansheng
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1985
|g 39(2023), 24 vom: 20. Juni, Seite 8404-8413
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnas
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| 773 |
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|g volume:39
|g year:2023
|g number:24
|g day:20
|g month:06
|g pages:8404-8413
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|u http://dx.doi.org/10.1021/acs.langmuir.3c00362
|3 Volltext
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