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231226s2023 xx |||||o 00| ||eng c |
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|a 10.1002/adma.202300180
|2 doi
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|a pubmed25n1190.xml
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|a (DE-627)NLM357328086
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|a (NLM)37230467
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|a DE-627
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|e rakwb
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|a eng
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| 100 |
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|a Chen, Zehao
|e verfasserin
|4 aut
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|a Mechanical Signal-Tailored Hydrogel Microspheres Recruit and Train Stem Cells for Precise Differentiation
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|c 2023
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|a Text
|b txt
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 05.10.2023
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|a Date Revised 05.10.2023
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2023 Wiley-VCH GmbH.
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|a The aberrant mechanical microenvironment in degenerated tissues induces misdirection of cell fate, making it challenging to achieve efficient endogenous regeneration. Herein, a hydrogel microsphere-based synthetic niche with integrated cell recruitment and targeted cell differentiation properties via mechanotransduction is constructed . Through the incorporation of microfluidics and photo-polymerization strategies, fibronectin (Fn) modified methacrylated gelatin (GelMA) microspheres are prepared with the independently tunable elastic modulus (1-10Kpa) and ligand density (2 and 10 µg mL-1 ), allowing a wide range of cytoskeleton modulation to trigger the corresponding mechanobiological signaling. The combination of the soft matrix (2Kpa) and low ligand density (2 µg mL-1 ) can support the nucleus pulposus (NP)-like differentiation of intervertebral disc (IVD) progenitor/stem cells by translocating Yes-associated protein (YAP), without the addition of inducible biochemical factors. Meanwhile, platelet-derived growth factor-BB (PDGF-BB) is loaded onto Fn-GelMA microspheres (PDGFFn-GelMA) via the heparin-binding domain of Fn to initiate endogenous cell recruitment. In in vivo experiments, hydrogel microsphere-niche maintained the IVD structure and stimulated matrix synthesis. Overall, this synthetic niche with cell recruiting and mechanical training capabilities offered a promising strategy for endogenous tissue regeneration
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|a Journal Article
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|a cell recruitment
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|a hydrogel microspheres
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|a mechanotransduction
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|a microfluidics
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|a stem cell differentiation
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|a Hydrogels
|2 NLM
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|a Ligands
|2 NLM
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|a Gelatin
|2 NLM
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|a 9000-70-8
|2 NLM
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| 700 |
1 |
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|a Lv, Zhendong
|e verfasserin
|4 aut
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|a Zhuang, Yaping
|e verfasserin
|4 aut
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| 700 |
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|a Saiding, Qimanguli
|e verfasserin
|4 aut
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| 700 |
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|a Yang, Wu
|e verfasserin
|4 aut
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|a Xiong, Wei
|e verfasserin
|4 aut
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|a Zhang, Zhen
|e verfasserin
|4 aut
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|a Chen, Hao
|e verfasserin
|4 aut
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|a Cui, Wenguo
|e verfasserin
|4 aut
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| 700 |
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|a Zhang, Yuhui
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Advanced materials (Deerfield Beach, Fla.)
|d 1998
|g 35(2023), 40 vom: 15. Okt., Seite e2300180
|w (DE-627)NLM098206397
|x 1521-4095
|7 nnas
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| 773 |
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|g volume:35
|g year:2023
|g number:40
|g day:15
|g month:10
|g pages:e2300180
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|u http://dx.doi.org/10.1002/adma.202300180
|3 Volltext
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