Self-Assembly of Peptide-Lipid Nanoparticles for the Efficient Delivery of Nucleic Acids

A transfection formulation is successfully developed to deliver nucleic acids by adding an auxiliary lipid (DOTAP) to the peptide, and the transfection efficiency of pDNA reaches 72.6%, which is close to Lipofectamine 2000. In addition, the designed KHL peptide-DOTAP complex exhibits good biocompati...

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Veröffentlicht in:Langmuir : the ACS journal of surfaces and colloids. - 1985. - 39(2023), 21 vom: 30. Mai, Seite 7484-7494
1. Verfasser: Zhang, Jiaojiao (VerfasserIn)
Weitere Verfasser: Wang, Zixuan, Min, Jiwei, Zhang, Xuelin, Su, Rongxin, Wang, Yuefei, Qi, Wei
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2023
Zugriff auf das übergeordnete Werk:Langmuir : the ACS journal of surfaces and colloids
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Lipid Nanoparticles Nucleic Acids 1,2-dioleoyloxy-3-(trimethylammonium)propane MR86K0XRQP Liposomes Peptides
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520 |a A transfection formulation is successfully developed to deliver nucleic acids by adding an auxiliary lipid (DOTAP) to the peptide, and the transfection efficiency of pDNA reaches 72.6%, which is close to Lipofectamine 2000. In addition, the designed KHL peptide-DOTAP complex exhibits good biocompatibility by cytotoxicity and hemolysis analysis. The mRNA delivery experiment indicates that the complex had a 9- or 10-fold increase compared with KHL or DOTAP alone. Intracellular localization shows that KHL/DOTAP can achieve good endolysosomal escape. Our design provides a new platform for improving the transfection efficiency of peptide vectors 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 7 |a Lipid Nanoparticles  |2 NLM 
650 7 |a Nucleic Acids  |2 NLM 
650 7 |a 1,2-dioleoyloxy-3-(trimethylammonium)propane  |2 NLM 
650 7 |a MR86K0XRQP  |2 NLM 
650 7 |a Liposomes  |2 NLM 
650 7 |a Peptides  |2 NLM 
700 1 |a Wang, Zixuan  |e verfasserin  |4 aut 
700 1 |a Min, Jiwei  |e verfasserin  |4 aut 
700 1 |a Zhang, Xuelin  |e verfasserin  |4 aut 
700 1 |a Su, Rongxin  |e verfasserin  |4 aut 
700 1 |a Wang, Yuefei  |e verfasserin  |4 aut 
700 1 |a Qi, Wei  |e verfasserin  |4 aut 
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