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231226s2023 xx |||||o 00| ||eng c |
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|a 10.1007/s10646-023-02627-w
|2 doi
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|a eng
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1 |
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|a Goldoni, Angélica
|e verfasserin
|4 aut
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|a Comet assay in Aegla platensis (Decapoda
|b Anomura) using a non-lethal hemolymph field sampling for in situ monitoring of freshwater genotoxicity
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|c 2023
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|a Text
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|2 rdacontent
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|a ƒaComputermedien
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|a ƒa Online-Ressource
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|a Date Completed 14.03.2023
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|a Date Revised 14.03.2023
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
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|a This study aimed to apply the comet assay on Aegla platensis crabs as a suitable non-destructive approach for in situ monitoring of freshwater genotoxicity. Animals were captured during four sampling periods in a stream under minor anthropogenic impacts in Southern Brazil. Crabs were captured with a hand net, then the hemolymph samples were collected, and the animals were released into the stream after a 20-min recovery time. Hemolymph samples were transported to the laboratory and used to perform the alkaline comet assay. Results showed an intermediate level in the DNA damage index (range 107.3-165.0 arbitrary unit). No significant differences were observed among the different sampling periods. Hemolymph was successfully used as a non-lethal source of biological samples, and the comet assay using A. platensis proved to be a feasible approach for genotoxicity studies
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|a Journal Article
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4 |
|a Biomarkers
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| 650 |
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4 |
|a Comet assay
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| 650 |
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4 |
|a Crustaceans
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| 650 |
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4 |
|a Genotoxicity
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| 650 |
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4 |
|a In situ monitoring
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1 |
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|a Pacheco, Marcelo Rech
|e verfasserin
|4 aut
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1 |
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|a da Silva, Luciano Basso
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Ecotoxicology (London, England)
|d 1992
|g 32(2023), 2 vom: 21. März, Seite 160-165
|w (DE-627)NLM098212214
|x 1573-3017
|7 nnas
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| 773 |
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|g volume:32
|g year:2023
|g number:2
|g day:21
|g month:03
|g pages:160-165
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