Comet assay in Aegla platensis (Decapoda : Anomura) using a non-lethal hemolymph field sampling for in situ monitoring of freshwater genotoxicity
© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
| Publié dans: | Ecotoxicology (London, England). - 1992. - 32(2023), 2 vom: 21. März, Seite 160-165 |
|---|---|
| Auteur principal: | |
| Autres auteurs: | , |
| Format: | Article en ligne |
| Langue: | English |
| Publié: |
2023
|
| Accès à la collection: | Ecotoxicology (London, England) |
| Sujets: | Journal Article Biomarkers Comet assay Crustaceans Genotoxicity In situ monitoring |
| Résumé: | © 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature. This study aimed to apply the comet assay on Aegla platensis crabs as a suitable non-destructive approach for in situ monitoring of freshwater genotoxicity. Animals were captured during four sampling periods in a stream under minor anthropogenic impacts in Southern Brazil. Crabs were captured with a hand net, then the hemolymph samples were collected, and the animals were released into the stream after a 20-min recovery time. Hemolymph samples were transported to the laboratory and used to perform the alkaline comet assay. Results showed an intermediate level in the DNA damage index (range 107.3-165.0 arbitrary unit). No significant differences were observed among the different sampling periods. Hemolymph was successfully used as a non-lethal source of biological samples, and the comet assay using A. platensis proved to be a feasible approach for genotoxicity studies |
|---|---|
| Description: | Date Completed 14.03.2023 Date Revised 14.03.2023 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1573-3017 |
| DOI: | 10.1007/s10646-023-02627-w |