The origin and distribution of 'Kokubu'-type splice-site mutations of the MLO genes in tobacco varieties

Copyright © 2022 by JAPANESE SOCIETY OF BREEDING.

Bibliographische Detailangaben
Veröffentlicht in:Breeding science. - 1998. - 72(2022), 3 vom: Juni, Seite 248-256
1. Verfasser: Arai, Masao (VerfasserIn)
Weitere Verfasser: Komatsu, Tomoyuki, Udagawa, Hisashi, Tajima, Tomoyuki, Sato, Seiki
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2022
Zugriff auf das übergeordnete Werk:Breeding science
Schlagworte:Journal Article Japanese domestic tobacco MILDEW LOCUS O cultivar ‘Kokubu’ leaf shape powdery mildew resistance splice-site mutations
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520 |a The Japanese domestic tobacco (Nicotiana tabacum L.) cultivar 'Kokubu' shows high powdery mildew resistance that is controlled by splice-site mutations of two MILDEW LOCUS O genes, NtMLO1 and NtMLO2. We investigated the existence of the same NtMLO1/2 splice mutations in the genomes of various tobacco varieties cultivated in Japan and other countries. In total, 14 Japanese domestic cultivars, which were mainly distributed in Kagoshima, had splice-site mutations in both NtMLO1 and NtMLO2. In addition, tobacco cultivars containing only the NtMLO1 splice-site mutation were found in various tobacco production areas in Japan, but no cultivars with only the NtMLO2 splice-site mutation were detected. Moreover, the NtMLO1 splice-site mutation was detected in native Asian, Oriental and cigar tobacco varieties. Consequently, we speculate that these powdery mildew-resistant tobacco cultivars were generated relative recently in the Kagoshima area when a spontaneous mutation occurred at the NtMLO2 splice site in a cultivar already containing the NtMLO1 splice-site mutation and that the NtMLO1 splice-site mutation occurred during the early period of tobacco seed dissemination from the Americas to Asia and Japan 
650 4 |a Journal Article 
650 4 |a Japanese domestic tobacco 
650 4 |a MILDEW LOCUS O 
650 4 |a cultivar ‘Kokubu’ 
650 4 |a leaf shape 
650 4 |a powdery mildew resistance 
650 4 |a splice-site mutations 
700 1 |a Komatsu, Tomoyuki  |e verfasserin  |4 aut 
700 1 |a Udagawa, Hisashi  |e verfasserin  |4 aut 
700 1 |a Tajima, Tomoyuki  |e verfasserin  |4 aut 
700 1 |a Sato, Seiki  |e verfasserin  |4 aut 
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773 1 8 |g volume:72  |g year:2022  |g number:3  |g month:06  |g pages:248-256 
856 4 0 |u http://dx.doi.org/10.1270/jsbbs.22001  |3 Volltext 
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