The origin and distribution of 'Kokubu'-type splice-site mutations of the MLO genes in tobacco varieties

The origin and distribution of 'Kokubu'-type splice-site mutations of the MLO genes in tobacco varieties

Copyright © 2022 by JAPANESE SOCIETY OF BREEDING.

Bibliographische Detailangaben
Veröffentlicht in:Breeding science. - 1998. - 72(2022), 3 vom: Juni, Seite 248-256
1. Verfasser: Arai, Masao (VerfasserIn)
Weitere Verfasser: Komatsu, Tomoyuki, Udagawa, Hisashi, Tajima, Tomoyuki, Sato, Seiki
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2022
Zugriff auf das übergeordnete Werk:Breeding science
Schlagworte:Journal Article Japanese domestic tobacco MILDEW LOCUS O cultivar ‘Kokubu’ leaf shape powdery mildew resistance splice-site mutations
Beschreibung
Zusammenfassung:Copyright © 2022 by JAPANESE SOCIETY OF BREEDING.
The Japanese domestic tobacco (Nicotiana tabacum L.) cultivar 'Kokubu' shows high powdery mildew resistance that is controlled by splice-site mutations of two MILDEW LOCUS O genes, NtMLO1 and NtMLO2. We investigated the existence of the same NtMLO1/2 splice mutations in the genomes of various tobacco varieties cultivated in Japan and other countries. In total, 14 Japanese domestic cultivars, which were mainly distributed in Kagoshima, had splice-site mutations in both NtMLO1 and NtMLO2. In addition, tobacco cultivars containing only the NtMLO1 splice-site mutation were found in various tobacco production areas in Japan, but no cultivars with only the NtMLO2 splice-site mutation were detected. Moreover, the NtMLO1 splice-site mutation was detected in native Asian, Oriental and cigar tobacco varieties. Consequently, we speculate that these powdery mildew-resistant tobacco cultivars were generated relative recently in the Kagoshima area when a spontaneous mutation occurred at the NtMLO2 splice site in a cultivar already containing the NtMLO1 splice-site mutation and that the NtMLO1 splice-site mutation occurred during the early period of tobacco seed dissemination from the Americas to Asia and Japan
Beschreibung:Date Revised 22.11.2022
published: Print-Electronic
Citation Status PubMed-not-MEDLINE
ISSN:1344-7610
DOI:10.1270/jsbbs.22001