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231226s2022 xx |||||o 00| ||eng c |
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|a 10.2144/btn-2022-0068
|2 doi
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|a pubmed24n1162.xml
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|e rakwb
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|a eng
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1 |
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|a Harikai, Naoki
|e verfasserin
|4 aut
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| 245 |
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|a Real-time PCR method for detection of short DNA using a deoxyuridine probe and application for detection of fomivirsen
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|c 2022
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 19.12.2022
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|a Date Revised 22.12.2022
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a This study sought to develop a short DNA detection method using a deoxyuridine probe and polymerase chain reaction. The probe was hybridized to the target short DNA, which was then extended by DNA polymerase. The extended DNA was used for real-time PCR after the probe was removed by uracil DNA glycosylase. This method measured from 0.01 to 10 nM of a model short DNA sequence of 17 nucleotides. The method was then used to detect the nucleic acid medicine fomivirsen, as well as 21 phosphorothioate nucleotides, and to quantify 0.1-100 nM of fomivirsen. This method may be useful for detecting short DNA fragments, such as functional nucleotides
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a fomivirsen
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|a nucleic acid medicine
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| 650 |
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|a polymerase chain reaction
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| 650 |
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|a short DNA
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|a uracil DNA glycosylase
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| 650 |
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|a fomivirsen
|2 NLM
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| 650 |
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|a QX5LK7YCHV
|2 NLM
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|a DNA
|2 NLM
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|a 9007-49-2
|2 NLM
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|a Thionucleotides
|2 NLM
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| 650 |
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|a Deoxyuridine
|2 NLM
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| 650 |
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7 |
|a W78I7AY22C
|2 NLM
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| 700 |
1 |
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|a Tanaka, Yuko
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Miyashita, Satoshi
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zaima, Kazumasa
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Shinomiya, Kazufusa
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t BioTechniques
|d 1988
|g 73(2022), 6 vom: 18. Dez., Seite 281-287
|w (DE-627)NLM012627046
|x 1940-9818
|7 nnns
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| 773 |
1 |
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|g volume:73
|g year:2022
|g number:6
|g day:18
|g month:12
|g pages:281-287
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|u http://dx.doi.org/10.2144/btn-2022-0068
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