Real-time PCR method for detection of short DNA using a deoxyuridine probe and application for detection of fomivirsen

This study sought to develop a short DNA detection method using a deoxyuridine probe and polymerase chain reaction. The probe was hybridized to the target short DNA, which was then extended by DNA polymerase. The extended DNA was used for real-time PCR after the probe was removed by uracil DNA glyco...

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Publié dans:	BioTechniques. - 1991. - 73(2022), 6 vom: 18. Dez., Seite 281-287
Auteur principal:	Harikai, Naoki (Auteur)
Autres auteurs:	Tanaka, Yuko, Miyashita, Satoshi, Zaima, Kazumasa, Shinomiya, Kazufusa
Format:	Article en ligne
Langue:	English
Publié:	2022
Accès à la collection:	BioTechniques
Sujets:	Journal Article Research Support, Non-U.S. Gov't fomivirsen nucleic acid medicine polymerase chain reaction short DNA uracil DNA glycosylase QX5LK7YCHV DNA 9007-49-2 plus... Thionucleotides Deoxyuridine W78I7AY22C