Real-time PCR method for detection of short DNA using a deoxyuridine probe and application for detection of fomivirsen
This study sought to develop a short DNA detection method using a deoxyuridine probe and polymerase chain reaction. The probe was hybridized to the target short DNA, which was then extended by DNA polymerase. The extended DNA was used for real-time PCR after the probe was removed by uracil DNA glyco...
Ausführliche Beschreibung
Bibliographische Detailangaben
| Veröffentlicht in: | BioTechniques. - 1988. - 73(2022), 6 vom: 18. Dez., Seite 281-287
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| 1. Verfasser: |
Harikai, Naoki
(VerfasserIn) |
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| Weitere Verfasser: |
Tanaka, Yuko,
Miyashita, Satoshi,
Zaima, Kazumasa,
Shinomiya, Kazufusa |
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| Format: | Online-Aufsatz
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| Sprache: | English |
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| Veröffentlicht: |
2022
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| Zugriff auf das übergeordnete Werk: | BioTechniques
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| Schlagworte: | Journal Article
Research Support, Non-U.S. Gov't
fomivirsen
nucleic acid medicine
polymerase chain reaction
short DNA
uracil DNA glycosylase
QX5LK7YCHV
DNA
9007-49-2
mehr...
Thionucleotides
Deoxyuridine
W78I7AY22C |
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