Efficient multiplexing and variant discrimination in reverse-transcription loop-mediated isothermal amplification with sequence-specific hybridization probes

Loop-mediated isothermal amplification (LAMP) has proven a robust and reliable nucleic acid amplification method that is well suited for simplified and rapid molecular diagnostics. Various approaches have emerged for sequence-specific detection of LAMP products, but with limitations to their widespr...

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Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1988. - 73(2022), 5 vom: 09. Nov., Seite 247-255
1. Verfasser: Zhang, Yinhua (VerfasserIn)
Weitere Verfasser: Tanner, Nathan A
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2022
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article Research Support, Non-U.S. Gov't LAMP RT-LAMP SNP discrimination isothermal amplification molecular diagnostics multiplexing variant identification RNA, Viral