Low-cost modular systems for agarose gel documentation

While conducting recombinant DNA technology procedures, such as DNA purification, agarose gel electrophoresis is often used for identification, characterization and quantification of DNA. The collection of data for experiments involving such techniques frequently involves capturing images using syst...

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Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1991. - 73(2022), 5 vom: 15. Nov., Seite 227-232
1. Verfasser: Gonzalez, Alfredo G (VerfasserIn)
Weitere Verfasser: González-García, Maribel, Pérez-Ballestero, Rafael
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2022
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Research Support, N.I.H., Extramural DNA Linux Raspberry Pi agarose gel education electrophoresis imaging mehr... open source smartphone webcam Sepharose 9012-36-6 9007-49-2
Beschreibung
Zusammenfassung:While conducting recombinant DNA technology procedures, such as DNA purification, agarose gel electrophoresis is often used for identification, characterization and quantification of DNA. The collection of data for experiments involving such techniques frequently involves capturing images using systems that are expensive and/or proprietary, such that they are not user-serviceable when they malfunction or become antiquated. In response to these limitations, work was done to replace the authors' existing aging Mac OS-based modular system with open-source software and generic hardware. Several versions of a modular imaging system that can be adjusted to fit nearly all use cases are described. The systems developed can accommodate diverse uses from research laboratories to educational environments where commercial systems could be unaffordable
Beschreibung:Date Completed 23.11.2022
Date Revised 04.12.2022
published: Print-Electronic
Citation Status MEDLINE
ISSN:1940-9818
DOI:10.2144/btn-2022-0060