Low-cost modular systems for agarose gel documentation

While conducting recombinant DNA technology procedures, such as DNA purification, agarose gel electrophoresis is often used for identification, characterization and quantification of DNA. The collection of data for experiments involving such techniques frequently involves capturing images using syst...

Ausführliche Beschreibung

Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1991. - 73(2022), 5 vom: 15. Nov., Seite 227-232
1. Verfasser: Gonzalez, Alfredo G (VerfasserIn)
Weitere Verfasser: González-García, Maribel, Pérez-Ballestero, Rafael
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2022
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Research Support, N.I.H., Extramural DNA Linux Raspberry Pi agarose gel education electrophoresis imaging mehr... open source smartphone webcam Sepharose 9012-36-6 9007-49-2
LEADER 01000caa a22002652c 4500
001 NLM348321945
003 DE-627
005 20250304012226.0
007 cr uuu---uuuuu
008 231226s2022 xx |||||o 00| ||eng c
024 7 |a 10.2144/btn-2022-0060  |2 doi 
028 5 2 |a pubmed25n1160.xml 
035 |a (DE-627)NLM348321945 
035 |a (NLM)36318177 
040 |a DE-627  |b ger  |c DE-627  |e rakwb 
041 |a eng 
100 1 |a Gonzalez, Alfredo G  |e verfasserin  |4 aut 
245 1 0 |a Low-cost modular systems for agarose gel documentation 
264 1 |c 2022 
336 |a Text  |b txt  |2 rdacontent 
337 |a ƒaComputermedien  |b c  |2 rdamedia 
338 |a ƒa Online-Ressource  |b cr  |2 rdacarrier 
500 |a Date Completed 23.11.2022 
500 |a Date Revised 04.12.2022 
500 |a published: Print-Electronic 
500 |a Citation Status MEDLINE 
520 |a While conducting recombinant DNA technology procedures, such as DNA purification, agarose gel electrophoresis is often used for identification, characterization and quantification of DNA. The collection of data for experiments involving such techniques frequently involves capturing images using systems that are expensive and/or proprietary, such that they are not user-serviceable when they malfunction or become antiquated. In response to these limitations, work was done to replace the authors' existing aging Mac OS-based modular system with open-source software and generic hardware. Several versions of a modular imaging system that can be adjusted to fit nearly all use cases are described. The systems developed can accommodate diverse uses from research laboratories to educational environments where commercial systems could be unaffordable 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a Research Support, N.I.H., Extramural 
650 4 |a DNA 
650 4 |a Linux 
650 4 |a Raspberry Pi 
650 4 |a agarose gel 
650 4 |a education 
650 4 |a electrophoresis 
650 4 |a imaging 
650 4 |a open source 
650 4 |a smartphone 
650 4 |a webcam 
650 7 |a Sepharose  |2 NLM 
650 7 |a 9012-36-6  |2 NLM 
650 7 |a DNA  |2 NLM 
650 7 |a 9007-49-2  |2 NLM 
700 1 |a González-García, Maribel  |e verfasserin  |4 aut 
700 1 |a Pérez-Ballestero, Rafael  |e verfasserin  |4 aut 
773 0 8 |i Enthalten in  |t BioTechniques  |d 1991  |g 73(2022), 5 vom: 15. Nov., Seite 227-232  |w (DE-627)NLM012627046  |x 1940-9818  |7 nnas 
773 1 8 |g volume:73  |g year:2022  |g number:5  |g day:15  |g month:11  |g pages:227-232 
856 4 0 |u http://dx.doi.org/10.2144/btn-2022-0060  |3 Volltext 
912 |a GBV_USEFLAG_A 
912 |a SYSFLAG_A 
912 |a GBV_NLM 
912 |a GBV_ILN_21 
912 |a GBV_ILN_22 
912 |a GBV_ILN_24 
912 |a GBV_ILN_39 
912 |a GBV_ILN_40 
912 |a GBV_ILN_50 
912 |a GBV_ILN_60 
912 |a GBV_ILN_62 
912 |a GBV_ILN_65 
912 |a GBV_ILN_70 
912 |a GBV_ILN_99 
912 |a GBV_ILN_121 
912 |a GBV_ILN_130 
912 |a GBV_ILN_227 
912 |a GBV_ILN_350 
912 |a GBV_ILN_618 
912 |a GBV_ILN_640 
912 |a GBV_ILN_754 
912 |a GBV_ILN_2001 
912 |a GBV_ILN_2002 
912 |a GBV_ILN_2003 
912 |a GBV_ILN_2005 
912 |a GBV_ILN_2006 
912 |a GBV_ILN_2007 
912 |a GBV_ILN_2008 
912 |a GBV_ILN_2009 
912 |a GBV_ILN_2010 
912 |a GBV_ILN_2012 
912 |a GBV_ILN_2015 
912 |a GBV_ILN_2018 
912 |a GBV_ILN_2023 
912 |a GBV_ILN_2035 
912 |a GBV_ILN_2040 
912 |a GBV_ILN_2060 
912 |a GBV_ILN_2099 
912 |a GBV_ILN_2105 
912 |a GBV_ILN_2121 
912 |a GBV_ILN_2470 
951 |a AR 
952 |d 73  |j 2022  |e 5  |b 15  |c 11  |h 227-232