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20231226030630.0 |
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231226s2022 xx |||||o 00| ||eng c |
| 024 |
7 |
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|a 10.1111/nph.18478
|2 doi
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|a pubmed24n1153.xml
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|a (DE-627)NLM346107105
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|a (NLM)36093737
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Cheng, Steven Le Hung
|e verfasserin
|4 aut
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| 245 |
1 |
0 |
|a Nutrient status regulates MED19a phase separation for ORESARA1-dependent senescence
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| 264 |
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|c 2022
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 03.02.2023
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|a Date Revised 03.02.2023
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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| 520 |
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|a © 2022 Temasek Life Sciences Laboratory. New Phytologist © 2022 New Phytologist Foundation.
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| 520 |
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|a The mediator complex is highly conserved in eukgaryotes and is integral for transcriptional responses. Mediator subunits associate with signal-responsive transcription factors (TF) to activate expression of specific signal-responsive genes. As the key TF of Arabidopsis thaliana senescence, ORESARA1 (ORE1) is required for nitrogen deficiency (-N) induced senescence; however, the mediator subunit that associates with ORE1 remains unknown. Here, we show that Arabidopsis MED19a associates with ORE1 to activate -N senescence-responsive genes. Disordered MED19a forms inducible nuclear condensates under -N that is regulated by decreasing MED19a lysine acetylation. MED19a carboxyl terminus (cMED19a) harbors a mixed-charged intrinsically disordered region (MC-IDR) required for ORE1 interaction and liquid-liquid phase separation (LLPS). Plant and human cMED19 are sufficient to form heterotypic condensates with ORE1. Human cMED19 MC-IDR, but not yeast cMED19 IDR, partially complements med19a suggesting functional conservation in evolutionarily distant eukaryotes. Phylogenetic analysis of eukaryotic cMED19 revealed that the MC-IDR could arise through convergent evolution. Our result of MED19 MC-IDR suggests that plant MED19 is regulated by phase separation during stress responses
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4 |
|a Journal Article
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| 650 |
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4 |
|a Research Support, Non-U.S. Gov't
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| 650 |
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4 |
|a liquid-liquid phase separation
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| 650 |
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4 |
|a mediator complex
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| 650 |
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4 |
|a nitrogen deficiency
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| 650 |
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4 |
|a senescence
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| 650 |
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4 |
|a transcriptional regulation
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| 650 |
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7 |
|a Arabidopsis Proteins
|2 NLM
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| 650 |
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7 |
|a AT5G39610 protein, Arabidopsis
|2 NLM
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| 650 |
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7 |
|a MED19 protein, human
|2 NLM
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| 650 |
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7 |
|a Mediator Complex
|2 NLM
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| 650 |
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7 |
|a Trans-Activators
|2 NLM
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| 650 |
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7 |
|a Transcription Factors
|2 NLM
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| 650 |
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7 |
|a AT5G12230 protein, Arabidopsis
|2 NLM
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| 700 |
1 |
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|a Wu, Hui-Wen
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Xu, Haiying
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Singh, Reuben Manjit
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Yao, Tao
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Jang, In-Cheol
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Chua, Nam-Hai
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t The New phytologist
|d 1979
|g 236(2022), 5 vom: 05. Dez., Seite 1779-1795
|w (DE-627)NLM09818248X
|x 1469-8137
|7 nnns
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| 773 |
1 |
8 |
|g volume:236
|g year:2022
|g number:5
|g day:05
|g month:12
|g pages:1779-1795
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| 856 |
4 |
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|u http://dx.doi.org/10.1111/nph.18478
|3 Volltext
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|a GBV_ILN_350
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|a AR
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|d 236
|j 2022
|e 5
|b 05
|c 12
|h 1779-1795
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