Nutrient status regulates MED19a phase separation for ORESARA1-dependent senescence
© 2022 Temasek Life Sciences Laboratory. New Phytologist © 2022 New Phytologist Foundation.
| Veröffentlicht in: | The New phytologist. - 1979. - 236(2022), 5 vom: 05. Dez., Seite 1779-1795 |
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| 1. Verfasser: | |
| Weitere Verfasser: | , , , , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2022
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| Zugriff auf das übergeordnete Werk: | The New phytologist |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't liquid-liquid phase separation mediator complex nitrogen deficiency senescence transcriptional regulation Arabidopsis Proteins AT5G39610 protein, Arabidopsis MED19 protein, human mehr... |
| Zusammenfassung: | © 2022 Temasek Life Sciences Laboratory. New Phytologist © 2022 New Phytologist Foundation. The mediator complex is highly conserved in eukgaryotes and is integral for transcriptional responses. Mediator subunits associate with signal-responsive transcription factors (TF) to activate expression of specific signal-responsive genes. As the key TF of Arabidopsis thaliana senescence, ORESARA1 (ORE1) is required for nitrogen deficiency (-N) induced senescence; however, the mediator subunit that associates with ORE1 remains unknown. Here, we show that Arabidopsis MED19a associates with ORE1 to activate -N senescence-responsive genes. Disordered MED19a forms inducible nuclear condensates under -N that is regulated by decreasing MED19a lysine acetylation. MED19a carboxyl terminus (cMED19a) harbors a mixed-charged intrinsically disordered region (MC-IDR) required for ORE1 interaction and liquid-liquid phase separation (LLPS). Plant and human cMED19 are sufficient to form heterotypic condensates with ORE1. Human cMED19 MC-IDR, but not yeast cMED19 IDR, partially complements med19a suggesting functional conservation in evolutionarily distant eukaryotes. Phylogenetic analysis of eukaryotic cMED19 revealed that the MC-IDR could arise through convergent evolution. Our result of MED19 MC-IDR suggests that plant MED19 is regulated by phase separation during stress responses |
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| Beschreibung: | Date Completed 03.02.2023 Date Revised 03.02.2023 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1469-8137 |
| DOI: | 10.1111/nph.18478 |