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231226s2022 xx |||||o 00| ||eng c |
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|a 10.1021/acs.langmuir.2c01540
|2 doi
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|a pubmed24n1523.xml
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|a (DE-627)NLM345132475
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|a (NLM)35994730
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Rahimi, Ehsan
|e verfasserin
|4 aut
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|a Morphological and Surface Potential Characterization of Protein Nanobiofilm Formation on Magnesium Alloy Oxide
|b Their Role in Biodegradation
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|c 2022
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 08.09.2022
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|a Date Revised 04.09.2024
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a The formation of a protein nanobiofilm on the surface of degradable biomaterials such as magnesium (Mg) and its alloys influences metal ion release, cell adhesion/spreading, and biocompatibility. During the early stage of human body implantation, competition and interaction between inorganic species and protein molecules result in a complex film containing Mg oxide and a protein layer. This film affects the electrochemical properties of the metal surface, the protein conformational arrangement, and the electronic properties of the protein/Mg oxide interface. In this study, we discuss the impact of various simulated body fluids, including sodium chloride (NaCl), phosphate-buffered saline (PBS), and Hanks' solutions on protein adsorption, electrochemical interactions, and electrical surface potential (ESP) distribution at the adsorbed protein/Mg oxide interface. After 10 min of immersion in NaCl, atomic force microscopy (AFM) and scanning Kelvin probe force microscopy (SKPFM) showed a higher surface roughness related to enhanced degradation and lower ESP distribution on a Mg-based alloy than those in other solutions. Furthermore, adding bovine serum albumin (BSA) to all solutions caused a decline in the total surface roughness and ESP magnitude on the Mg alloy surface, particularly in the NaCl electrolyte. Using SKPFM surface analysis, we detected a protein nanobiofilm (∼10-20 nm) with an aggregated and/or fibrillary morphology only on the Mg surface exposed in Hanks' and PBS solutions; these surfaces had a lower ESP value than the oxide layer
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Alloys
|2 NLM
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|a Oxides
|2 NLM
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|a Magnesium Oxide
|2 NLM
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|a 3A3U0GI71G
|2 NLM
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|a Sodium Chloride
|2 NLM
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|a 451W47IQ8X
|2 NLM
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| 650 |
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|a Magnesium
|2 NLM
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| 650 |
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|a I38ZP9992A
|2 NLM
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| 700 |
1 |
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|a Imani, Amin
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Lekka, Maria
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Andreatta, Francesco
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Gonzalez-Garcia, Yaiza
|e verfasserin
|4 aut
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| 700 |
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|a Mol, Johannes M C
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Asselin, Edouard
|e verfasserin
|4 aut
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| 700 |
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|a Fedrizzi, Lorenzo
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1999
|g 38(2022), 35 vom: 06. Sept., Seite 10854-10866
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
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|g volume:38
|g year:2022
|g number:35
|g day:06
|g month:09
|g pages:10854-10866
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|u http://dx.doi.org/10.1021/acs.langmuir.2c01540
|3 Volltext
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|d 38
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|h 10854-10866
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