Integration of multi-omics data reveals interplay between brassinosteroid and Target of Rapamycin Complex signaling in Arabidopsis

Bibliographische Detailangaben
Veröffentlicht in:	The New phytologist. - 1979. - 236(2022), 3 vom: 08. Nov., Seite 893-910
1. Verfasser:	Montes, Christian (VerfasserIn)
Weitere Verfasser:	Wang, Ping, Liao, Ching-Yi, Nolan, Trevor M, Song, Gaoyuan, Clark, Natalie M, Elmore, J Mitch, Guo, Hongqing, Bassham, Diane C, Yin, Yanhai, Walley, Justin W
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2022
Zugriff auf das übergeordnete Werk:	The New phytologist
Schlagworte:	Journal Article Research Support, N.I.H., Extramural Research Support, U.S. Gov't, Non-P.H.S. Research Support, Non-U.S. Gov't BIN2 RAP TOR TORAutophagy brassinosteroids integrative mehr... multi-omics network Arabidopsis Proteins Brassinosteroids Transcription Factors Protein Kinases EC 2.7.- BIN2 protein, Arabidopsis EC 2.7.1.- Sirolimus W36ZG6FT64

Beschreibung
Zusammenfassung:	© 2022 The Authors. New Phytologist © 2022 New Phytologist Foundation. Brassinosteroids (BRs) and Target of Rapamycin Complex (TORC) are two major actors coordinating plant growth and stress responses. Brassinosteroids function through a signaling pathway to extensively regulate gene expression and TORC is known to regulate translation and autophagy. Recent studies have revealed connections between these two pathways, but a system-wide view of their interplay is still missing. We quantified the level of 23 975 transcripts, 11 183 proteins, and 27 887 phosphorylation sites in wild-type Arabidopsis thaliana and in mutants with altered levels of either BRASSINOSTEROID INSENSITIVE 2 (BIN2) or REGULATORY ASSOCIATED PROTEIN OF TOR 1B (RAPTOR1B), two key players in BR and TORC signaling, respectively. We found that perturbation of BIN2 or RAPTOR1B levels affects a common set of gene-products involved in growth and stress responses. Furthermore, we used the multi-omic data to reconstruct an integrated signaling network. We screened 41 candidate genes identified from the reconstructed network and found that loss of function mutants of many of these proteins led to an altered BR response and/or modulated autophagy activity. Altogether, these results establish a predictive network that defines different layers of molecular interactions between BR- or TORC-regulated growth and autophagy
Beschreibung:	Date Completed 07.10.2022 Date Revised 04.01.2023 published: Print-Electronic Citation Status MEDLINE
ISSN:	1469-8137
DOI:	10.1111/nph.18404