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231226s2022 xx |||||o 00| ||eng c |
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|a 10.1111/vcp.13156
|2 doi
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|a pubmed25n1146.xml
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|a (DE-627)NLM344030075
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|a (NLM)35883213
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Harris, R Adam
|e verfasserin
|4 aut
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|a Detection and Characterization of Paraproteinemia in Canine Chronic B-cell Lymphocytic Leukemia Using Routine and Free Light Chain Immunofixation
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|c 2022
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 02.12.2022
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|a Date Revised 26.04.2024
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2022 The Authors. Veterinary Clinical Pathology published by Wiley Periodicals LLC on behalf of American Society for Veterinary Clinical Pathology.
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|a BACKGROUND: Hyperglobulinemia is reported in 26% of canine chronic B-cell lymphocytic leukemia (B-CLL) cases. However, few cases have been characterized by protein electrophoresis and immunofixation (IF), and the incidence of a monoclonal protein (M-protein) is unknown using these techniques
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|a OBJECTIVE: To characterize and determine the proportion of canine B-CLL cases with an M-protein using plasma protein electrophoresis (PPE), routine and free light chain (fLC) IF, and to assess if productive B-CLL cases express MUM1/IRF4 by cell tube block (CTB)
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|a METHODS: PPE, routine (targeting IgG, IgA, IgM, IgG4, and light chain) and fLC IF were performed using 48 dog B-CLL plasma samples from patients diagnosed via peripheral blood flow cytometry. CTB was performed on a separate cohort of 15 patients
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|a RESULTS: Hyperproteinemia (>7.5 g/dL) was present in 17/48 cases (35%). An M-protein was detected in 32/48 cases (67%). Of these, 19/32 cases (59%) had only complete (monoclonal heavy and light chain) M-proteins detected, 10/32 cases (31%) had both complete and fLC M-proteins detected, and 3/32 cases (9%) had only an fLC M-protein detected. IgM was the most common clonal immunoglobulin isotype detected (23 cases). CD21+ cell counts were higher in cases with detectable M-protein. Plasma fLC IF suggested β-γ region interference, likely caused by clotting proteins. All B-CLL cases consistently expressed PAX5 and did not express MUM1/IRF4
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|a CONCLUSIONS: Most B-CLL cases had an M-protein and were not hyperproteinemic. Most cases with paraproteins had a complete IgM monoclonal gammopathy; a subset had documented fLCs. The prognostic significance of heavy and fLC presence should be evaluated
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|a Journal Article
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|a dog
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|a electrophoresis
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|a gammopathy
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| 650 |
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|a hyperglobulinemia
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| 650 |
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|a hyperproteinemia
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| 650 |
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|a monoclonal
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| 650 |
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|a Immunoglobulin Light Chains
|2 NLM
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| 650 |
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|a Immunoglobulin M
|2 NLM
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| 700 |
1 |
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|a Gary, Erik B
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Rout, Emily D
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Avery, Anne C
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Moore, A Russell
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Veterinary clinical pathology
|d 1975
|g 51(2022), 4 vom: 06. Dez., Seite 551-559
|w (DE-627)NLM098159984
|x 1939-165X
|7 nnas
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| 773 |
1 |
8 |
|g volume:51
|g year:2022
|g number:4
|g day:06
|g month:12
|g pages:551-559
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| 856 |
4 |
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|u http://dx.doi.org/10.1111/vcp.13156
|3 Volltext
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|d 51
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|e 4
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|c 12
|h 551-559
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