Abscisic acid-controlled redox proteome of Arabidopsis and its regulation by heterotrimeric Gβ protein

Bibliographische Detailangaben
Veröffentlicht in:	The New phytologist. - 1979. - 236(2022), 2 vom: 06. Okt., Seite 447-463
1. Verfasser:	Smythers, Amanda L (VerfasserIn)
Weitere Verfasser:	Bhatnagar, Nikita, Ha, Chien, Majumdar, Parinita, McConnell, Evan W, Mohanasundaram, Boominathan, Hicks, Leslie M, Pandey, Sona
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2022
Zugriff auf das übergeordnete Werk:	The New phytologist
Schlagworte:	Journal Article Research Support, U.S. Gov't, Non-P.H.S. AGB1 Arabidopsis G-protein abscisic acid photosynthesis proteomics redox redox proteomics mehr... AGB1 protein, Arabidopsis Arabidopsis Proteins GTP-Binding Protein beta Subunits Plant Growth Regulators Proteome Reactive Oxygen Species Abscisic Acid 72S9A8J5GW Heterotrimeric GTP-Binding Proteins EC 3.6.5.1 Cysteine K848JZ4886

Beschreibung
Zusammenfassung:	© 2022 The Authors. New Phytologist © 2022 New Phytologist Foundation. The plant hormone abscisic acid (ABA) plays crucial roles in regulation of stress responses and growth modulation. Heterotrimeric G-proteins are key mediators of ABA responses. Both ABA and G-proteins have also been implicated in intracellular redox regulation; however, the extent to which reversible protein oxidation manipulates ABA and/or G-protein signaling remains uncharacterized. To probe the role of reversible protein oxidation in plant stress response and its dependence on G-proteins, we determined the ABA-dependent reversible redoxome of wild-type and Gβ-protein null mutant agb1 of Arabidopsis. We quantified 6891 uniquely oxidized cysteine-containing peptides, 923 of which show significant changes in oxidation following ABA treatment. The majority of these changes required the presence of G-proteins. Divergent pathways including primary metabolism, reactive oxygen species response, translation and photosynthesis exhibited both ABA- and G-protein-dependent redox changes, many of which occurred on proteins not previously linked to them. We report the most comprehensive ABA-dependent plant redoxome and uncover a complex network of reversible oxidations that allow ABA and G-proteins to rapidly adjust cellular signaling to adapt to changing environments. Physiological validation of a subset of these observations suggests that functional G-proteins are required to maintain intracellular redox homeostasis and fully execute plant stress responses
Beschreibung:	Date Completed 28.09.2022 Date Revised 20.10.2022 published: Print-Electronic Citation Status MEDLINE
ISSN:	1469-8137
DOI:	10.1111/nph.18348