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231226s2022 xx |||||o 00| ||eng c |
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|a 10.1111/jfd.13674
|2 doi
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|a pubmed24n1142.xml
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|a (DE-627)NLM342791974
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|a DE-627
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|e rakwb
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|a eng
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| 100 |
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|a Li, Yuan
|e verfasserin
|4 aut
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|a Establishment of an astrocyte-like cell line from the brain of tilapia (Oreochromis niloticus) for virus pathogenesis and a vitro model of the blood-brain barrier
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|c 2022
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 15.09.2022
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|a Date Revised 15.09.2022
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2022 John Wiley & Sons Ltd.
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|a A new cell line was established from the brain of a cultured fish, tilapia (Oreochromis niloticus), designated as TA-02 (Tilapia Astrocyte clone 02 cell line). The TA-02 cells are grown for 300 days in an L-15 medium supplemented with 10% fetal bovine serum (FBS). This cell line showed excellent proliferative capacity and expressed various neuroglial cell markers, including SOX2, SOX10, Hes1, Notch1, Occludin, E-cadherin, and GFAP. In addition, TA-02 cells were susceptible to Tilapia Lake Virus (TiLV) as demonstrated by the presence of a severe cytopathic effect (CPE), virus particle in a transmission electron microscope (TEM), and PCR positive signal. Bacterial cytotoxicity studies showed that Streptococcus agalactiae was toxic to TA-02 cells. When co-culture with trans-well, TA-02 exhibited prominent barrier properties, manifested by tight intercellular junctions and increased trans-endothelial electrical resistance (TEER). In addition, the barrier is effective against Escherichia coli (non-meningitis pathogenic bacteria). In contrast, S. agalactiae (meningitis pathogenic bacteria) can pass through the membrane comprising the cells in the trans-well insert. The newly established TA-02 cell line provided a valuable tool for virus pathogenesis and a vitro model of the fish blood-brain barrier
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|a Journal Article
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|a Oreochromis niloticus
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|a astrocyte
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|a blood-brain barrier
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|a cell line
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| 650 |
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4 |
|a trans-well
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| 700 |
1 |
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|a Huang, Yu
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Cai, Jia
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Jiang, Dongneng
|e verfasserin
|4 aut
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| 700 |
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|a Jian, Ji-Chang
|e verfasserin
|4 aut
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| 700 |
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|a Lu, Yi-Shan
|e verfasserin
|4 aut
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| 700 |
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|a Wang, Bei
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Journal of fish diseases
|d 1998
|g 45(2022), 10 vom: 03. Okt., Seite 1451-1462
|w (DE-627)NLM098166034
|x 1365-2761
|7 nnns
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| 773 |
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|g volume:45
|g year:2022
|g number:10
|g day:03
|g month:10
|g pages:1451-1462
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|u http://dx.doi.org/10.1111/jfd.13674
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