Establishment of an astrocyte-like cell line from the brain of tilapia (Oreochromis niloticus) for virus pathogenesis and a vitro model of the blood-brain barrier
© 2022 John Wiley & Sons Ltd.
| Veröffentlicht in: | Journal of fish diseases. - 1998. - 45(2022), 10 vom: 03. Okt., Seite 1451-1462 |
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| 1. Verfasser: | |
| Weitere Verfasser: | , , , , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2022
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| Zugriff auf das übergeordnete Werk: | Journal of fish diseases |
| Schlagworte: | Journal Article Oreochromis niloticus astrocyte blood-brain barrier cell line trans-well |
| Zusammenfassung: | © 2022 John Wiley & Sons Ltd. A new cell line was established from the brain of a cultured fish, tilapia (Oreochromis niloticus), designated as TA-02 (Tilapia Astrocyte clone 02 cell line). The TA-02 cells are grown for 300 days in an L-15 medium supplemented with 10% fetal bovine serum (FBS). This cell line showed excellent proliferative capacity and expressed various neuroglial cell markers, including SOX2, SOX10, Hes1, Notch1, Occludin, E-cadherin, and GFAP. In addition, TA-02 cells were susceptible to Tilapia Lake Virus (TiLV) as demonstrated by the presence of a severe cytopathic effect (CPE), virus particle in a transmission electron microscope (TEM), and PCR positive signal. Bacterial cytotoxicity studies showed that Streptococcus agalactiae was toxic to TA-02 cells. When co-culture with trans-well, TA-02 exhibited prominent barrier properties, manifested by tight intercellular junctions and increased trans-endothelial electrical resistance (TEER). In addition, the barrier is effective against Escherichia coli (non-meningitis pathogenic bacteria). In contrast, S. agalactiae (meningitis pathogenic bacteria) can pass through the membrane comprising the cells in the trans-well insert. The newly established TA-02 cell line provided a valuable tool for virus pathogenesis and a vitro model of the fish blood-brain barrier |
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| Beschreibung: | Date Completed 15.09.2022 Date Revised 15.09.2022 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1365-2761 |
| DOI: | 10.1111/jfd.13674 |