Unprocessed wheat γ-gliadin reduces gluten accumulation associated with the endoplasmic reticulum stress and elevated cell death

Unprocessed wheat γ-gliadin reduces gluten accumulation associated with the endoplasmic reticulum stress and elevated cell death

© 2022 The Authors. New Phytologist © 2022 New Phytologist Foundation.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 236(2022), 1 vom: 26. Okt., Seite 146-164
1. Verfasser: Chen, Qian (VerfasserIn)
Weitere Verfasser: Yang, Changfeng, Zhang, Zhaoheng, Wang, Zihao, Chen, Yongming, Rossi, Vincenzo, Chen, Wei, Xin, Mingming, Su, Zhenqi, Du, Jinkun, Guo, Weilong, Hu, Zhaorong, Liu, Jie, Peng, Huiru, Ni, Zhongfu, Sun, Qixin, Yao, Yingyin
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2022
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Research Support, Non-U.S. Gov't ER stress cell death low-gluten seed storage protein wheat Glutens 8002-80-0 Gliadin 9007-90-3
Beschreibung
Zusammenfassung:© 2022 The Authors. New Phytologist © 2022 New Phytologist Foundation.
Along with increasing demands for high yield, elite processing quality and improved nutrient value in wheat, concerns have emerged around the effects of gluten in wheat-based foods on human health. However, knowledge of the mechanisms regulating gluten accumulation remains largely unexplored. Here we report the identification and characterization of a wheat low gluten protein 1 (lgp1) mutant that shows extremely low levels of gliadins and glutenins. The lgp1 mutation in a single γ-gliadin gene causes defective signal peptide cleavage, resulting in the accumulation of an excessive amount of unprocessed γ-gliadin and a reduced level of gluten, which alters the endoplasmic reticulum (ER) structure, forms the autophagosome-like structures, leads to the delivery of seed storage proteins to the extracellular space and causes a reduction in starch biosynthesis. Physiologically, these effects trigger ER stress and cell death. This study unravels a unique mechanism that unprocessed γ-gliadin reduces gluten accumulation associated with ER stress and elevated cell death in wheat. Moreover, the reduced gluten level in the lgp1 mutant makes it a good candidate for specific diets for patients with diabetes or kidney diease
Beschreibung:Date Completed 09.09.2022
Date Revised 05.09.2024
published: Print-Electronic
Citation Status MEDLINE
ISSN:1469-8137
DOI:10.1111/nph.18316