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231226s2022 xx |||||o 00| ||eng c |
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|a 10.1093/jxb/erac248
|2 doi
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|a pubmed24n1139.xml
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|a (DE-627)NLM34183100X
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|a (NLM)35661206
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Feng, Meng-Qi
|e verfasserin
|4 aut
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| 245 |
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|a miR156 regulates somatic embryogenesis by modulating starch accumulation in citrus
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|c 2022
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 20.10.2022
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|a Date Revised 21.10.2022
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|a published: Print
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|a Citation Status MEDLINE
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|a © The Author(s) 2022. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissionsoup.com.
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|a Somatic embryogenesis (SE) is a major regeneration approach for in vitro cultured tissues of plants, including citrus. However, SE capability is difficult to maintain, and recalcitrance to SE has become a major obstacle to plant biotechnology. We previously reported that miR156-SPL modules regulate SE in citrus callus. However, the downstream regulatory pathway of the miR156-SPL module in SE remains unclear. In this study, we found that transcription factors CsAGL15 and CsFUS3 bind to the CsMIR156A promoter and activate its expression. Suppression of csi-miR156a function leads to up-regulation of four target genes, SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (CsSPL) genes, and reduction of SE efficiency. In the short tandem target mimic (STTM)-miR156a overexpression callus (MIM156), the number of amyloplasts and starch content were significantly reduced, and genes involved in starch synthesis and transport were down-regulated. csi-miR172d was down-regulated, whereas the target genes, CsTOE1.1 and CsTOE1.2, which inhibit the expression of starch biosynthesis genes, were up-regulated. In our working model, CsAGL15 and CsFUS3 activate csi-miR156a, which represses CsSPLs and further regulates csi-miR172d and CsTOEs, thus altering starch accumulation in callus cells and regulating SE in citrus. This study elucidates the pathway of miR156-SPLs and miR172-TOEs-mediated regulation of SE, and provides new insights into enhancing SE capability in citrus
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Callus
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|a citrus
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|a miR156
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|a miR172
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| 650 |
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|a somatic embryogenesis
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| 650 |
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|a starch
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|a Starch
|2 NLM
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| 650 |
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|a 9005-25-8
|2 NLM
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| 650 |
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|a MicroRNAs
|2 NLM
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| 650 |
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|a Transcription Factors
|2 NLM
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| 700 |
1 |
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|a Lu, Meng-Di
|e verfasserin
|4 aut
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| 700 |
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|a Long, Jian-Mei
|e verfasserin
|4 aut
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| 700 |
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|a Yin, Zhao-Ping
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Jiang, Nan
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wang, Peng-Bo
|e verfasserin
|4 aut
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| 700 |
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|a Liu, Yue
|e verfasserin
|4 aut
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|a Guo, Wen-Wu
|e verfasserin
|4 aut
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| 700 |
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|a Wu, Xiao-Meng
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 73(2022), 18 vom: 18. Okt., Seite 6170-6185
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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| 773 |
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|g volume:73
|g year:2022
|g number:18
|g day:18
|g month:10
|g pages:6170-6185
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| 856 |
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|u http://dx.doi.org/10.1093/jxb/erac248
|3 Volltext
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|d 73
|j 2022
|e 18
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|h 6170-6185
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