miR156 regulates somatic embryogenesis by modulating starch accumulation in citrus

Bibliographische Detailangaben
Veröffentlicht in:	Journal of experimental botany. - 1985. - 73(2022), 18 vom: 18. Okt., Seite 6170-6185
1. Verfasser:	Feng, Meng-Qi (VerfasserIn)
Weitere Verfasser:	Lu, Meng-Di, Long, Jian-Mei, Yin, Zhao-Ping, Jiang, Nan, Wang, Peng-Bo, Liu, Yue, Guo, Wen-Wu, Wu, Xiao-Meng
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2022
Zugriff auf das übergeordnete Werk:	Journal of experimental botany
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't Callus citrus miR156 miR172 somatic embryogenesis starch Starch 9005-25-8 mehr... MicroRNAs Transcription Factors

Beschreibung
Zusammenfassung:	© The Author(s) 2022. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissionsoup.com. Somatic embryogenesis (SE) is a major regeneration approach for in vitro cultured tissues of plants, including citrus. However, SE capability is difficult to maintain, and recalcitrance to SE has become a major obstacle to plant biotechnology. We previously reported that miR156-SPL modules regulate SE in citrus callus. However, the downstream regulatory pathway of the miR156-SPL module in SE remains unclear. In this study, we found that transcription factors CsAGL15 and CsFUS3 bind to the CsMIR156A promoter and activate its expression. Suppression of csi-miR156a function leads to up-regulation of four target genes, SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (CsSPL) genes, and reduction of SE efficiency. In the short tandem target mimic (STTM)-miR156a overexpression callus (MIM156), the number of amyloplasts and starch content were significantly reduced, and genes involved in starch synthesis and transport were down-regulated. csi-miR172d was down-regulated, whereas the target genes, CsTOE1.1 and CsTOE1.2, which inhibit the expression of starch biosynthesis genes, were up-regulated. In our working model, CsAGL15 and CsFUS3 activate csi-miR156a, which represses CsSPLs and further regulates csi-miR172d and CsTOEs, thus altering starch accumulation in callus cells and regulating SE in citrus. This study elucidates the pathway of miR156-SPLs and miR172-TOEs-mediated regulation of SE, and provides new insights into enhancing SE capability in citrus
Beschreibung:	Date Completed 20.10.2022 Date Revised 21.10.2022 published: Print Citation Status MEDLINE
ISSN:	1460-2431
DOI:	10.1093/jxb/erac248