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231226s2022 xx |||||o 00| ||eng c |
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|a 10.1111/vcp.13056
|2 doi
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|a pubmed24n1138.xml
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|a (DE-627)NLM341668834
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|a (NLM)35644839
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|a DE-627
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|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Etzioni, Athema L
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Osteosarcoma diagnosed in a dog using a formalin-fixed fine-needle aspirate biopsy
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1 |
|c 2022
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 14.09.2022
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|a Date Revised 14.09.2022
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2022 American Society for Veterinary Clinical Pathology.
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|a BACKGROUND: Fine-needle aspirate (FNA) biopsy is considered a quick technique to access and identify the cell types present in a pathologic lesion or make a diagnosis. Often, clinicians want to know if they are dealing with an inflammatory lesion with or without infectious agents or a neoplastic lesion. At times, neoplastic lesions may be confounded by the presence of inflammatory cells
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|a OBJECTIVES: We aimed to evaluate the application of a formalin-fixed FNA, designated the cytologic/histopathologic (CytoHisto) technique, to determine the diagnostic quality and ability to arrive at a definitive diagnosis without the use of concentrated cell block or invasive full tissue biopsy procedures during sample collection
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|a METHODS: A 10-cc syringe with a 22-gauge 1-inch needle attached was used to obtain a sizable FNA biopsy sample from a thigh mass in a dog. The needle was removed from the syringe, and the material was expulsed from the syringe directly into 10% buffered formalin. After 24-48-h fixation, a strainer was used to facilitate placement of the fixed granular material into a micromesh biopsy processing/embedding cassette, and the sample was processed as a routine histopathology sample. A microtome was used to make thin sections stained with H&E initially. Then, subsequent sections were stained with immunohistochemical (IHC) stains vimentin, MUM1, and CD18. Alkaline phosphatase staining was performed on a previously Wright's-stained cytology following IHC results. All sections were coverslipped and viewed under a light microscope
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|a RESULTS: When unable to perform incisional or excisional biopsies, this CytoHisto, FNA in formalin technique was useful for collection and subsequent processing as a histopathology sample, with sectioning and then staining with H&E and IHC stains. Neoplastic cells were strongly immunoreactive for vimentin but negative for MUM1 and CD18. Scattered leukocytes within the background stained positively with CD18
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|a CONCLUSIONS: The CytoHisto technique is minimally invasive and allows for sectioning similar to a full-thickness excisional or incisional biopsy with subsequent H&E and IHC staining, and special stains allow for a definitive diagnosis of osteosarcoma. The CytoHisto technique is a practical diagnostic technique to pursue in clinical practice that minimizes patient invasiveness and maximizes sample collection time, similar to the routine FNA technique
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|a Journal Article
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4 |
|a CytoHisto
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| 650 |
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4 |
|a FNA biopsy
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| 650 |
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4 |
|a bone neoplasia
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| 650 |
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4 |
|a canine
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| 650 |
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4 |
|a cerclage wire
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| 650 |
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4 |
|a diagnostic technique
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| 650 |
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7 |
|a Vimentin
|2 NLM
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| 650 |
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7 |
|a Formaldehyde
|2 NLM
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| 650 |
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7 |
|a 1HG84L3525
|2 NLM
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| 773 |
0 |
8 |
|i Enthalten in
|t Veterinary clinical pathology
|d 1975
|g 51(2022), 3 vom: 15. Sept., Seite 349-355
|w (DE-627)NLM098159984
|x 1939-165X
|7 nnns
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| 773 |
1 |
8 |
|g volume:51
|g year:2022
|g number:3
|g day:15
|g month:09
|g pages:349-355
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| 856 |
4 |
0 |
|u http://dx.doi.org/10.1111/vcp.13056
|3 Volltext
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