Osteosarcoma diagnosed in a dog using a formalin-fixed fine-needle aspirate biopsy
© 2022 American Society for Veterinary Clinical Pathology.
| Veröffentlicht in: | Veterinary clinical pathology. - 1975. - 51(2022), 3 vom: 15. Sept., Seite 349-355 |
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| 1. Verfasser: | |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2022
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| Zugriff auf das übergeordnete Werk: | Veterinary clinical pathology |
| Schlagworte: | Journal Article CytoHisto FNA biopsy bone neoplasia canine cerclage wire diagnostic technique Vimentin Formaldehyde 1HG84L3525 |
| Zusammenfassung: | © 2022 American Society for Veterinary Clinical Pathology. BACKGROUND: Fine-needle aspirate (FNA) biopsy is considered a quick technique to access and identify the cell types present in a pathologic lesion or make a diagnosis. Often, clinicians want to know if they are dealing with an inflammatory lesion with or without infectious agents or a neoplastic lesion. At times, neoplastic lesions may be confounded by the presence of inflammatory cells OBJECTIVES: We aimed to evaluate the application of a formalin-fixed FNA, designated the cytologic/histopathologic (CytoHisto) technique, to determine the diagnostic quality and ability to arrive at a definitive diagnosis without the use of concentrated cell block or invasive full tissue biopsy procedures during sample collection METHODS: A 10-cc syringe with a 22-gauge 1-inch needle attached was used to obtain a sizable FNA biopsy sample from a thigh mass in a dog. The needle was removed from the syringe, and the material was expulsed from the syringe directly into 10% buffered formalin. After 24-48-h fixation, a strainer was used to facilitate placement of the fixed granular material into a micromesh biopsy processing/embedding cassette, and the sample was processed as a routine histopathology sample. A microtome was used to make thin sections stained with H&E initially. Then, subsequent sections were stained with immunohistochemical (IHC) stains vimentin, MUM1, and CD18. Alkaline phosphatase staining was performed on a previously Wright's-stained cytology following IHC results. All sections were coverslipped and viewed under a light microscope RESULTS: When unable to perform incisional or excisional biopsies, this CytoHisto, FNA in formalin technique was useful for collection and subsequent processing as a histopathology sample, with sectioning and then staining with H&E and IHC stains. Neoplastic cells were strongly immunoreactive for vimentin but negative for MUM1 and CD18. Scattered leukocytes within the background stained positively with CD18 CONCLUSIONS: The CytoHisto technique is minimally invasive and allows for sectioning similar to a full-thickness excisional or incisional biopsy with subsequent H&E and IHC staining, and special stains allow for a definitive diagnosis of osteosarcoma. The CytoHisto technique is a practical diagnostic technique to pursue in clinical practice that minimizes patient invasiveness and maximizes sample collection time, similar to the routine FNA technique |
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| Beschreibung: | Date Completed 14.09.2022 Date Revised 14.09.2022 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1939-165X |
| DOI: | 10.1111/vcp.13056 |