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231226s2022 xx |||||o 00| ||eng c |
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|a 10.1093/jxb/erac171
|2 doi
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|a pubmed24n1134.xml
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|a (DE-627)NLM340397292
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|a (NLM)35511088
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Shi, Weiling
|e verfasserin
|4 aut
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| 245 |
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|a The transcription factor StTINY3 enhances cold-induced sweetening resistance by coordinating starch resynthesis and sucrose hydrolysis in potato
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|c 2022
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 12.08.2022
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|a Date Revised 09.09.2022
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|a published: Print
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|a Citation Status MEDLINE
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|a © The Author(s) 2022. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissionsoup.com.
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|a The accumulation of reducing sugars in cold-stored tubers, known as cold-induced sweetening (CIS), negatively affects potato processing quality. The starch to sugar interconversion pathways that are altered in cold-stored CIS tubers have been elucidated, but the mechanism that regulates them remains largely unknown. This study identified a CBF/DREB transcription factor (StTINY3) that enhances CIS resistance by both activating starch biosynthesis and repressing the hydrolysis of sucrose to reducing sugars in detached cold-stored tubers. Silencing StTINY3 in a CIS-resistant genotype decreased CIS resistance, while overexpressing StTINY3 in a CIS-sensitive genotype increased CIS resistance, and altering StTINY3 expression was associated with expression changes in starch resynthesis-related genes. We showed first that overexpressing StTINY3 inhibited sucrose hydrolysis by enhancing expression of the invertase inhibitor gene StInvInh2, and second that StTINY3 promoted starch resynthesis by up-regulating a large subunit of the ADP-glucose pyrophosphorylase gene StAGPaseL3, and the glucose-6-phosphate transporter gene StG6PT2. Using electrophoretic mobility shift assays, we revealed that StTINY3 is a nuclear-localized transcriptional activator that directly binds to the dehydration-responsive element/CRT cis-element in the promoters of StInvInh2 and StAGPaseL3. Taken together, these findings established that StTINY3 influences CIS resistance in cold-stored tubers by coordinately modulating the starch to sugar interconversion pathways and is a good target for improving potato processing quality
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4 |
|a Journal Article
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4 |
|a Research Support, Non-U.S. Gov't
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| 650 |
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4 |
|a StAGPaseL3
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4 |
|a StInvInh2
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4 |
|a Cold-induced sweetening resistance
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4 |
|a StTINY3
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|a potato
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4 |
|a transcriptional regulation
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| 650 |
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|a Carbohydrates
|2 NLM
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| 650 |
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7 |
|a Plant Proteins
|2 NLM
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| 650 |
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|a Sugars
|2 NLM
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| 650 |
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|a Transcription Factors
|2 NLM
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| 650 |
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|a Sucrose
|2 NLM
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| 650 |
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|a 57-50-1
|2 NLM
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| 650 |
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|a Starch
|2 NLM
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| 650 |
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7 |
|a 9005-25-8
|2 NLM
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| 700 |
1 |
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|a Ma, Qiuqin
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Yin, Wang
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Liu, Tiantian
|e verfasserin
|4 aut
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| 700 |
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|a Song, Yuhao
|e verfasserin
|4 aut
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| 700 |
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|a Chen, Yuanya
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Song, Linjin
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Sun, Hui
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Hu, Shuting
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Liu, Tengfei
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Jiang, Rui
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Lv, Dianqiu
|e verfasserin
|4 aut
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| 700 |
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|a Song, Botao
|e verfasserin
|4 aut
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| 700 |
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|a Wang, Jichun
|e verfasserin
|4 aut
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| 700 |
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|a Liu, Xun
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 73(2022), 14 vom: 11. Aug., Seite 4968-4980
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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| 773 |
1 |
8 |
|g volume:73
|g year:2022
|g number:14
|g day:11
|g month:08
|g pages:4968-4980
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| 856 |
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|u http://dx.doi.org/10.1093/jxb/erac171
|3 Volltext
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|d 73
|j 2022
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