MicroRNA172 controls inflorescence meristem size through regulation of APETALA2 in Arabidopsis

MicroRNA172 controls inflorescence meristem size through regulation of APETALA2 in Arabidopsis

© 2022 The Authors New Phytologist © 2022 New Phytologist Foundation.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 235(2022), 1 vom: 18. Juli, Seite 356-371
1. Verfasser: Sang, Qing (VerfasserIn)
Weitere Verfasser: Vayssières, Alice, Ó'Maoiléidigh, Diarmuid S, Yang, Xia, Vincent, Coral, Bertran Garcia de Olalla, Enric, Cerise, Martina, Franzen, Rainer, Coupland, George
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2022
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Arabidopsis thaliana AUXIN RESPONSE FACTOR 3 (ARF3) flower determinacy flowering shoot apical meristem APETALA2 protein, Arabidopsis Arabidopsis Proteins Homeodomain Proteins mehr... MicroRNAs Mirn172 microRNA, Arabidopsis Nuclear Proteins
Beschreibung
Zusammenfassung:© 2022 The Authors New Phytologist © 2022 New Phytologist Foundation.
The APETALA2 (AP2) transcription factor regulates flower development, floral transition and shoot apical meristem (SAM) maintenance in Arabidopsis. AP2 is also regulated at the post-transcriptional level by microRNA172 (miR172), but the contribution of this to SAM maintenance is poorly understood. We generated transgenic plants carrying a form of AP2 that is resistant to miR172 (rAP2) or carrying a wild-type AP2 susceptible to miR172. Phenotypic and genetic analyses were performed on these lines and mir172 mutants to study the role of AP2 regulation by miR172 on meristem size and the rate of flower production. We found that rAP2 enlarges the inflorescence meristem by increasing cell size and cell number. Misexpression of rAP2 from heterologous promoters showed that AP2 acts in the central zone (CZ) and organizing center (OC) to increase SAM size. Furthermore, we found that AP2 is negatively regulated by AUXIN RESPONSE FACTOR 3 (ARF3). However, genetic analyses indicated that ARF3 also influences SAM size and flower production rate independently of AP2. The study identifies miR172/AP2 as a regulatory module controlling inflorescence meristem size and suggests that transcriptional regulation of AP2 by ARF3 fine-tunes SAM size determination
Beschreibung:Date Completed 03.06.2022
Date Revised 27.06.2022
published: Print-Electronic
Citation Status MEDLINE
ISSN:1469-8137
DOI:10.1111/nph.18111