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231225s2022 xx |||||o 00| ||eng c |
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|a 10.1002/adma.202110054
|2 doi
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|a pubmed24n1123.xml
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|a (DE-627)NLM33698474X
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|a (NLM)35166410
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Bernal, Paulina Nuñez
|e verfasserin
|4 aut
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|a Volumetric Bioprinting of Organoids and Optically Tuned Hydrogels to Build Liver-Like Metabolic Biofactories
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|c 2022
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 15.04.2022
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|a Date Revised 15.04.2022
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2022 The Authors. Advanced Materials published by Wiley-VCH GmbH.
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|a Organ- and tissue-level biological functions are intimately linked to microscale cell-cell interactions and to the overarching tissue architecture. Together, biofabrication and organoid technologies offer the unique potential to engineer multi-scale living constructs, with cellular microenvironments formed by stem cell self-assembled structures embedded in customizable bioprinted geometries. This study introduces the volumetric bioprinting of complex organoid-laden constructs, which capture key functions of the human liver. Volumetric bioprinting via optical tomography shapes organoid-laden gelatin hydrogels into complex centimeter-scale 3D structures in under 20 s. Optically tuned bioresins enable refractive index matching of specific intracellular structures, countering the disruptive impact of cell-mediated light scattering on printing resolution. This layerless, nozzle-free technique poses no harmful mechanical stresses on organoids, resulting in superior viability and morphology preservation post-printing. Bioprinted organoids undergo hepatocytic differentiation showing albumin synthesis, liver-specific enzyme activity, and remarkably acquired native-like polarization. Organoids embedded within low stiffness gelatins (<2 kPa) are bioprinted into mathematically defined lattices with varying degrees of pore network tortuosity, and cultured under perfusion. These structures act as metabolic biofactories in which liver-specific ammonia detoxification can be enhanced by the architectural profile of the constructs. This technology opens up new possibilities for regenerative medicine and personalized drug testing
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|a Journal Article
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|a biofabrication
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|a bioresins
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|a hydrogels
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|a light-based 3D printing
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|a volumetric additive manufacturing
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|a Hydrogels
|2 NLM
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|a Gelatin
|2 NLM
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|a 9000-70-8
|2 NLM
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|a Bouwmeester, Manon
|e verfasserin
|4 aut
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|a Madrid-Wolff, Jorge
|e verfasserin
|4 aut
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| 700 |
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|a Falandt, Marc
|e verfasserin
|4 aut
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|a Florczak, Sammy
|e verfasserin
|4 aut
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|a Rodriguez, Nuria Ginés
|e verfasserin
|4 aut
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|a Li, Yang
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|4 aut
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|a Größbacher, Gabriel
|e verfasserin
|4 aut
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|a Samsom, Roos-Anne
|e verfasserin
|4 aut
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|a van Wolferen, Monique
|e verfasserin
|4 aut
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|a van der Laan, Luc J W
|e verfasserin
|4 aut
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|a Delrot, Paul
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|4 aut
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|a Loterie, Damien
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|4 aut
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|a Malda, Jos
|e verfasserin
|4 aut
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|a Moser, Christophe
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|a Spee, Bart
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|a Levato, Riccardo
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Advanced materials (Deerfield Beach, Fla.)
|d 1998
|g 34(2022), 15 vom: 01. Apr., Seite e2110054
|w (DE-627)NLM098206397
|x 1521-4095
|7 nnns
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|g volume:34
|g year:2022
|g number:15
|g day:01
|g month:04
|g pages:e2110054
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|u http://dx.doi.org/10.1002/adma.202110054
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