Arabidopsis thaliana CYCLIC NUCLEOTIDE-GATED CHANNEL2 mediates extracellular ATP signal transduction in root epidermis

Arabidopsis thaliana CYCLIC NUCLEOTIDE-GATED CHANNEL2 mediates extracellular ATP signal transduction in root epidermis

© 2022 The Authors New Phytologist © 2022 New Phytologist Foundation.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 234(2022), 2 vom: 28. Apr., Seite 412-421
1. Verfasser: Wang, Limin (VerfasserIn)
Weitere Verfasser: Ning, Youzheng, Sun, Jian, Wilkins, Katie A, Matthus, Elsa, McNelly, Rose E, Dark, Adeeba, Rubio, Lourdes, Moeder, Wolfgang, Yoshioka, Keiko, Véry, Anne-Aliénor, Stacey, Gary, Leblanc-Fournier, Nathalie, Legué, Valérie, Moulia, Bruno, Davies, Julia M
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2022
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Arabidopsis CNGC4 CYCLIC NUCLEOTIDE-GATED CHANNEL2 (CNGC2) calcium depolarization extracellular ATP plasma membrane potential mehr... root epidermis Arabidopsis Proteins Cyclic Nucleotide-Gated Cation Channels Nucleotides, Cyclic Adenosine Triphosphate 8L70Q75FXE Calcium SY7Q814VUP
LEADER 01000naa a22002652 4500
001 NLM336101848
003 DE-627
005 20231225231354.0
007 cr uuu---uuuuu
008 231225s2022 xx |||||o 00| ||eng c
024 7 |a 10.1111/nph.17987  |2 doi 
028 5 2 |a pubmed24n1120.xml 
035 |a (DE-627)NLM336101848 
035 |a (NLM)35075689 
040 |a DE-627  |b ger  |c DE-627  |e rakwb 
041 |a eng 
100 1 |a Wang, Limin  |e verfasserin  |4 aut 
245 1 0 |a Arabidopsis thaliana CYCLIC NUCLEOTIDE-GATED CHANNEL2 mediates extracellular ATP signal transduction in root epidermis 
264 1 |c 2022 
336 |a Text  |b txt  |2 rdacontent 
337 |a ƒaComputermedien  |b c  |2 rdamedia 
338 |a ƒa Online-Ressource  |b cr  |2 rdacarrier 
500 |a Date Completed 31.03.2022 
500 |a Date Revised 31.07.2022 
500 |a published: Print-Electronic 
500 |a Citation Status MEDLINE 
520 |a © 2022 The Authors New Phytologist © 2022 New Phytologist Foundation. 
520 |a Damage can be signalled by extracellular ATP (eATP) using plasma membrane (PM) receptors to effect cytosolic free calcium ion ([Ca2+ ]cyt ) increase as a second messenger. The downstream PM Ca2+ channels remain enigmatic. Here, the Arabidopsis thaliana Ca2+ channel subunit CYCLIC NUCLEOTIDE-GATED CHANNEL2 (CNGC2) was identified as a critical component linking eATP receptors to downstream [Ca2+ ]cyt signalling in roots. Extracellular ATP-induced changes in single epidermal cell PM voltage and conductance were measured electrophysiologically, changes in root [Ca2+ ]cyt were measured with aequorin, and root transcriptional changes were determined by quantitative real-time PCR. Two cngc2 loss-of-function mutants were used: cngc2-3 and defence not death1 (which expresses cytosolic aequorin). Extracellular ATP-induced transient depolarization of Arabidopsis root elongation zone epidermal PM voltage was Ca2+ dependent, requiring CNGC2 but not CNGC4 (its channel co-subunit in immunity signalling). Activation of PM Ca2+ influx currents also required CNGC2. The eATP-induced [Ca2+ ]cyt increase and transcriptional response in cngc2 roots were significantly impaired. CYCLIC NUCLEOTIDE-GATED CHANNEL2 is required for eATP-induced epidermal Ca2+ influx, causing depolarization leading to [Ca2+ ]cyt increase and damage-related transcriptional response 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a Research Support, U.S. Gov't, Non-P.H.S. 
650 4 |a Arabidopsis 
650 4 |a CNGC4 
650 4 |a CYCLIC NUCLEOTIDE-GATED CHANNEL2 (CNGC2) 
650 4 |a calcium 
650 4 |a depolarization 
650 4 |a extracellular ATP 
650 4 |a plasma membrane potential 
650 4 |a root epidermis 
650 7 |a Arabidopsis Proteins  |2 NLM 
650 7 |a Cyclic Nucleotide-Gated Cation Channels  |2 NLM 
650 7 |a Nucleotides, Cyclic  |2 NLM 
650 7 |a Adenosine Triphosphate  |2 NLM 
650 7 |a 8L70Q75FXE  |2 NLM 
650 7 |a Calcium  |2 NLM 
650 7 |a SY7Q814VUP  |2 NLM 
700 1 |a Ning, Youzheng  |e verfasserin  |4 aut 
700 1 |a Sun, Jian  |e verfasserin  |4 aut 
700 1 |a Wilkins, Katie A  |e verfasserin  |4 aut 
700 1 |a Matthus, Elsa  |e verfasserin  |4 aut 
700 1 |a McNelly, Rose E  |e verfasserin  |4 aut 
700 1 |a Dark, Adeeba  |e verfasserin  |4 aut 
700 1 |a Rubio, Lourdes  |e verfasserin  |4 aut 
700 1 |a Moeder, Wolfgang  |e verfasserin  |4 aut 
700 1 |a Yoshioka, Keiko  |e verfasserin  |4 aut 
700 1 |a Véry, Anne-Aliénor  |e verfasserin  |4 aut 
700 1 |a Stacey, Gary  |e verfasserin  |4 aut 
700 1 |a Leblanc-Fournier, Nathalie  |e verfasserin  |4 aut 
700 1 |a Legué, Valérie  |e verfasserin  |4 aut 
700 1 |a Moulia, Bruno  |e verfasserin  |4 aut 
700 1 |a Davies, Julia M  |e verfasserin  |4 aut 
773 0 8 |i Enthalten in  |t The New phytologist  |d 1979  |g 234(2022), 2 vom: 28. Apr., Seite 412-421  |w (DE-627)NLM09818248X  |x 1469-8137  |7 nnns 
773 1 8 |g volume:234  |g year:2022  |g number:2  |g day:28  |g month:04  |g pages:412-421 
856 4 0 |u http://dx.doi.org/10.1111/nph.17987  |3 Volltext 
912 |a GBV_USEFLAG_A 
912 |a SYSFLAG_A 
912 |a GBV_NLM 
912 |a GBV_ILN_350 
951 |a AR 
952 |d 234  |j 2022  |e 2  |b 28  |c 04  |h 412-421