Arabidopsis thaliana CYCLIC NUCLEOTIDE-GATED CHANNEL2 mediates extracellular ATP signal transduction in root epidermis

Bibliographische Detailangaben
Veröffentlicht in:	The New phytologist. - 1979. - 234(2022), 2 vom: 28. Apr., Seite 412-421
1. Verfasser:	Wang, Limin (VerfasserIn)
Weitere Verfasser:	Ning, Youzheng, Sun, Jian, Wilkins, Katie A, Matthus, Elsa, McNelly, Rose E, Dark, Adeeba, Rubio, Lourdes, Moeder, Wolfgang, Yoshioka, Keiko, Véry, Anne-Aliénor, Stacey, Gary, Leblanc-Fournier, Nathalie, Legué, Valérie, Moulia, Bruno, Davies, Julia M
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2022
Zugriff auf das übergeordnete Werk:	The New phytologist
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Arabidopsis CNGC4 CYCLIC NUCLEOTIDE-GATED CHANNEL2 (CNGC2) calcium depolarization extracellular ATP plasma membrane potential mehr... root epidermis Arabidopsis Proteins Cyclic Nucleotide-Gated Cation Channels Nucleotides, Cyclic Adenosine Triphosphate 8L70Q75FXE Calcium SY7Q814VUP

Beschreibung
Zusammenfassung:	© 2022 The Authors New Phytologist © 2022 New Phytologist Foundation. Damage can be signalled by extracellular ATP (eATP) using plasma membrane (PM) receptors to effect cytosolic free calcium ion ([Ca2+ ]cyt ) increase as a second messenger. The downstream PM Ca2+ channels remain enigmatic. Here, the Arabidopsis thaliana Ca2+ channel subunit CYCLIC NUCLEOTIDE-GATED CHANNEL2 (CNGC2) was identified as a critical component linking eATP receptors to downstream [Ca2+ ]cyt signalling in roots. Extracellular ATP-induced changes in single epidermal cell PM voltage and conductance were measured electrophysiologically, changes in root [Ca2+ ]cyt were measured with aequorin, and root transcriptional changes were determined by quantitative real-time PCR. Two cngc2 loss-of-function mutants were used: cngc2-3 and defence not death1 (which expresses cytosolic aequorin). Extracellular ATP-induced transient depolarization of Arabidopsis root elongation zone epidermal PM voltage was Ca2+ dependent, requiring CNGC2 but not CNGC4 (its channel co-subunit in immunity signalling). Activation of PM Ca2+ influx currents also required CNGC2. The eATP-induced [Ca2+ ]cyt increase and transcriptional response in cngc2 roots were significantly impaired. CYCLIC NUCLEOTIDE-GATED CHANNEL2 is required for eATP-induced epidermal Ca2+ influx, causing depolarization leading to [Ca2+ ]cyt increase and damage-related transcriptional response
Beschreibung:	Date Completed 31.03.2022 Date Revised 31.07.2022 published: Print-Electronic Citation Status MEDLINE
ISSN:	1469-8137
DOI:	10.1111/nph.17987