Proteomic analysis reveals responsive mechanisms for saline-alkali stress in alfalfa

Copyright © 2021 Elsevier Masson SAS. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Plant physiology and biochemistry : PPB. - 1991. - 170(2022) vom: 01. Jan., Seite 146-159
1. Verfasser: Ling, Lei (VerfasserIn)
Weitere Verfasser: An, Yimin, Wang, Dan, Tang, Lu, Du, Binghao, Shu, Yongjun, Bai, Yan, Guo, Changhong
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2022
Zugriff auf das übergeordnete Werk:Plant physiology and biochemistry : PPB
Schlagworte:Journal Article Differentially abundant proteins (DAPs) Integrative analysis Medicago sativa Proteome Alkalies Plant Proteins
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520 |a Saline-alkali stress is a major abiotic stress that limits plant growth, yield, and geographical distribution. Alfalfa is a perennial legume with the largest planting area in the world because of its high protein content, good palatability, and long utilization life. However, saline-alkali stress seriously affects alfalfa yield and quality. To better understand the saline-alkali stress response mechanisms of alfalfa, an isobaric tags proteomics method was used to compare and analyse alfalfa under saline-alkali stress for 0, 1, and 7 days, and 126 (1 vs. 0 days) and 1869 (7 vs. 0 days) differentially abundant proteins (DAPs) were found. Through integrative analysis with differentially expressed genes (DEGs), we found correlated DEGs-DAPs of RNA and protein with similar expression trends at the mRNA and protein levels; these were mainly involved in ABA and Ca2+ signal pathways, regulation of photosynthesis, ROS scavenging, secondary metabolism, and transcription factors (TFs) related to saline-alkali stress. Some genes not exhibiting such trends may have been regulated post-transcriptionally. Furthermore, through transgenic experiments, MsFTL was found to significantly improve the saline-alkali tolerance of plants. Overall, our findings provide important clues for understanding the molecular mechanisms underlying the response of alfalfa to saline-alkali stress 
650 4 |a Journal Article 
650 4 |a Differentially abundant proteins (DAPs) 
650 4 |a Integrative analysis 
650 4 |a Medicago sativa 
650 4 |a Proteome 
650 7 |a Alkalies  |2 NLM 
650 7 |a Plant Proteins  |2 NLM 
700 1 |a An, Yimin  |e verfasserin  |4 aut 
700 1 |a Wang, Dan  |e verfasserin  |4 aut 
700 1 |a Tang, Lu  |e verfasserin  |4 aut 
700 1 |a Du, Binghao  |e verfasserin  |4 aut 
700 1 |a Shu, Yongjun  |e verfasserin  |4 aut 
700 1 |a Bai, Yan  |e verfasserin  |4 aut 
700 1 |a Guo, Changhong  |e verfasserin  |4 aut 
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773 1 8 |g volume:170  |g year:2022  |g day:01  |g month:01  |g pages:146-159 
856 4 0 |u http://dx.doi.org/10.1016/j.plaphy.2021.12.003  |3 Volltext 
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