Proteomic analysis reveals responsive mechanisms for saline-alkali stress in alfalfa
Copyright © 2021 Elsevier Masson SAS. All rights reserved.
| Veröffentlicht in: | Plant physiology and biochemistry : PPB. - 1991. - 170(2022) vom: 01. Jan., Seite 146-159 |
|---|---|
| 1. Verfasser: | |
| Weitere Verfasser: | , , , , , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2022
|
| Zugriff auf das übergeordnete Werk: | Plant physiology and biochemistry : PPB |
| Schlagworte: | Journal Article Differentially abundant proteins (DAPs) Integrative analysis Medicago sativa Proteome Alkalies Plant Proteins |
| Zusammenfassung: | Copyright © 2021 Elsevier Masson SAS. All rights reserved. Saline-alkali stress is a major abiotic stress that limits plant growth, yield, and geographical distribution. Alfalfa is a perennial legume with the largest planting area in the world because of its high protein content, good palatability, and long utilization life. However, saline-alkali stress seriously affects alfalfa yield and quality. To better understand the saline-alkali stress response mechanisms of alfalfa, an isobaric tags proteomics method was used to compare and analyse alfalfa under saline-alkali stress for 0, 1, and 7 days, and 126 (1 vs. 0 days) and 1869 (7 vs. 0 days) differentially abundant proteins (DAPs) were found. Through integrative analysis with differentially expressed genes (DEGs), we found correlated DEGs-DAPs of RNA and protein with similar expression trends at the mRNA and protein levels; these were mainly involved in ABA and Ca2+ signal pathways, regulation of photosynthesis, ROS scavenging, secondary metabolism, and transcription factors (TFs) related to saline-alkali stress. Some genes not exhibiting such trends may have been regulated post-transcriptionally. Furthermore, through transgenic experiments, MsFTL was found to significantly improve the saline-alkali tolerance of plants. Overall, our findings provide important clues for understanding the molecular mechanisms underlying the response of alfalfa to saline-alkali stress |
|---|---|
| Beschreibung: | Date Completed 11.01.2022 Date Revised 11.01.2022 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1873-2690 |
| DOI: | 10.1016/j.plaphy.2021.12.003 |