Graphene Oxide-Supported Microwell Grids for Preparing Cryo-EM Samples with Controlled Ice Thickness

Bibliographische Detailangaben
Veröffentlicht in:	Advanced materials (Deerfield Beach, Fla.). - 1998. - 33(2021), 43 vom: 27. Okt., Seite e2102991
1. Verfasser:	Kang, Min-Ho (VerfasserIn)
Weitere Verfasser:	Park, Junsun, Kang, Sungsu, Jeon, Sungho, Lee, Minyoung, Shim, Ji-Yeon, Lee, Jeeyoung, Jeon, Tae Jin, Ahn, Min Kyung, Lee, Sung Mi, Kwon, Ohkyung, Kim, Byung Hyo, Meyerson, Joel R, Lee, Min Jae, Lim, Kwang-Il, Roh, Soung-Hun, Lee, Won Chul, Park, Jungwon
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2021
Zugriff auf das übergeordnete Werk:	Advanced materials (Deerfield Beach, Fla.)
Schlagworte:	Journal Article cryogenic-electron microscope graphene oxide microelectromechanical systems nanomaterials vitreous ice thickness Graphite 7782-42-5 Apoferritins 9013-31-4 mehr... Ice Fructose-Bisphosphate Aldolase EC 4.1.2.13 Silicon Compounds Chaperonin 60 silicon nitride QHB8T06IDK tau Proteins

Beschreibung
Zusammenfassung:	© 2021 Wiley-VCH GmbH. Cryogenic-electron microscopy (cryo-EM) is the preferred method to determine 3D structures of proteins and to study diverse material systems that intrinsically have radiation or air sensitivity. Current cryo-EM sample preparation methods provide limited control over the sample quality, which limits the efficiency and high throughput of 3D structure analysis. This is partly because it is difficult to control the thickness of the vitreous ice that embeds specimens, in the range of nanoscale, depending on the size and type of materials of interest. Thus, there is a need for fine regulation of the thickness of vitreous ice to deliver consistent high signal-to-noise ratios for low-contrast biological specimens. Herein, an advanced silicon-chip-based device is developed which has a regular array of micropatterned holes with a graphene oxide (GO) window on freestanding silicon nitride (Six Ny ). Accurately regulated depths of micropatterned holes enable precise control of vitreous ice thickness. Furthermore, GO window with affinity for biomolecules can facilitate concentration of the sample molecules at a higher level. Incorporation of micropatterned chips with a GO window enhances cryo-EM imaging for various nanoscale biological samples including human immunodeficiency viral particles, groEL tetradecamers, apoferritin octahedral, aldolase homotetramer complexes, and tau filaments, as well as inorganic materials
Beschreibung:	Date Completed 24.07.2024 Date Revised 24.07.2024 published: Print-Electronic Citation Status MEDLINE
ISSN:	1521-4095
DOI:	10.1002/adma.202102991