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20231225204652.0 |
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231225s2021 xx |||||o 00| ||eng c |
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|a 10.1007/s10646-021-02459-6
|2 doi
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|a pubmed24n1097.xml
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|a (DE-627)NLM329231502
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|a (NLM)34379241
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a de Souza, Irisdoris Rodrigues
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Development of 3D cultures of zebrafish liver and embryo cell lines
|b a comparison of different spheroid formation methods
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|c 2021
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 02.11.2021
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|a Date Revised 02.11.2021
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2021. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
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|a Fish cell spheroids are promising 3D culture models for vertebrate replacement in ecotoxicology. However, new alternative ecotoxicological methods must be adapted for applications in industry and for regulatory purposes; such methods must be cost-effective, simple to manipulate and provide rapid results. Therefore, we compared the effectiveness of the traditional hanging drop (HD), orbital shaking (OS), and HD combined with OS (HD+OS) methods on the formation of zebrafish cell line spheroids (ZFL and ZEM2S). Time in HD (3-5 days) and different 96-well plates [flat-bottom or ultra-low attachment of round-bottom (ULA-plates)] in OS were evaluated. Easy handling, rapid spheroid formation, uniform-sized spheroids, and circularity were assessed to identify the best spheroid protocol. Traditional HD alone did not result in ZFL spheroid formation, whereas HD (5 days)+OS did. When using the OS, spheroids only formed on the ULA-plate. Both HD+OS and OS were reproducible in size (177.50 ± 2.81 µm and 225.62 ± 19.20 µm, respectively) and circularity (0.83 ± 0.02 and 0.80 ± 0.01, respectively) of ZFL spheroids. Nevertheless, HD+OS required a considerable time to completely form spheroids (10 days) and intensive handling, whereas the OS was fast (5 days of incubation) and simple. OS also yielded reproducible ZEM2S spheroids in 1 day (226.23 ± 0.57 µm diameter and 0.80 ± 0.01 circularity). In conclusion, OS in ULA-plate is an effective and simple spheroid protocol for high-throughput ecotoxicity testing. This study contributes to identify a fast, reproducible, and simple protocol of single piscine spheroid formation in 96-well plates and supports the application of fish 3D model in industry and academia
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|a Journal Article
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|a 3D cell culture
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|a Alternative methods
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|a Ecotoxicology
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| 650 |
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|a Fish cell lines
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| 650 |
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|a Hanging drop
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| 650 |
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4 |
|a Orbital shaking
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| 700 |
1 |
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|a Canavez, Andrezza Di Pietro Micali
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Schuck, Desiree Cigaran
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Gagosian, Viviana Stephanie Costa
|e verfasserin
|4 aut
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| 700 |
1 |
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|a de Souza, Isisdoris Rodrigues
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Vicari, Taynah
|e verfasserin
|4 aut
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| 700 |
1 |
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|a da Silva Trindade, Edvaldo
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Cestari, Marta Margarete
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Lorencini, Marcio
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Leme, Daniela Morais
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Ecotoxicology (London, England)
|d 1992
|g 30(2021), 9 vom: 11. Nov., Seite 1893-1909
|w (DE-627)NLM098212214
|x 1573-3017
|7 nnns
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| 773 |
1 |
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|g volume:30
|g year:2021
|g number:9
|g day:11
|g month:11
|g pages:1893-1909
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|u http://dx.doi.org/10.1007/s10646-021-02459-6
|3 Volltext
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|a AR
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|d 30
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|h 1893-1909
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