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cr uuu---uuuuu |
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231225s2021 xx |||||o 00| ||eng c |
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|a 10.2144/btn-2021-0039
|2 doi
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|a pubmed25n1097.xml
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|a (DE-627)NLM329184059
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|a (NLM)34374327
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Cheng, Junhao
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Rapid Golden Gate assembly of exons from genomic DNA for protein expression in Escherichia coli and Pichia pastoris
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|c 2021
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 20.12.2021
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|a Date Revised 20.12.2021
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a The development of a quick, single-step cloning system for generation of multiexon gene expression constructs is presented. The system allows efficient and cost-effective assembly of multiple exons of interest genes into different expression plasmids in both Escherichia coli and Pichia pastoris. The high cloning efficiency and low cost of the system make it ideal for a novel workflow for the assembly of intron-bearing genes for expression in two different expression hosts
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Escherichia coli
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|a Golden Gate
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|a Pichia pastoris
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|a heterologous expression
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| 650 |
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4 |
|a quick exon assembly
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| 650 |
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|a Recombinant Proteins
|2 NLM
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| 650 |
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|a DNA
|2 NLM
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| 650 |
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7 |
|a 9007-49-2
|2 NLM
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| 700 |
1 |
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|a Wu, Mingkun
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhong, Ren
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Si, Dayong
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Meng, Geng
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhang, Rijun
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhang, Yueping
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t BioTechniques
|d 1993
|g 71(2021), 2 vom: 01. Aug., Seite 445-450
|w (DE-627)NLM012627046
|x 1940-9818
|7 nnas
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| 773 |
1 |
8 |
|g volume:71
|g year:2021
|g number:2
|g day:01
|g month:08
|g pages:445-450
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| 856 |
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|u http://dx.doi.org/10.2144/btn-2021-0039
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