Rapid Golden Gate assembly of exons from genomic DNA for protein expression in Escherichia coli and Pichia pastoris
The development of a quick, single-step cloning system for generation of multiexon gene expression constructs is presented. The system allows efficient and cost-effective assembly of multiple exons of interest genes into different expression plasmids in both Escherichia coli and Pichia pastoris. The...
Ausführliche Beschreibung
Bibliographische Detailangaben
| Veröffentlicht in: | BioTechniques. - 1993. - 71(2021), 2 vom: 01. Aug., Seite 445-450
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| 1. Verfasser: |
Cheng, Junhao
(VerfasserIn) |
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| Weitere Verfasser: |
Wu, Mingkun,
Zhong, Ren,
Si, Dayong,
Meng, Geng,
Zhang, Rijun,
Zhang, Yueping |
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| Format: | Online-Aufsatz
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| Sprache: | English |
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| Veröffentlicht: |
2021
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| Zugriff auf das übergeordnete Werk: | BioTechniques
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| Schlagworte: | Journal Article
Research Support, Non-U.S. Gov't
Escherichia coli
Golden Gate
Pichia pastoris
heterologous expression
quick exon assembly
Recombinant Proteins
DNA
9007-49-2 |
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