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|a 10.2144/btn-2021-0017
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|a eng
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1 |
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|a Xu, Tingting
|e verfasserin
|4 aut
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1 |
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|a Screening for androgen agonists using autonomously bioluminescent HEK293 reporter cells
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|c 2021
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|a Text
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|a Date Completed 20.12.2021
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|a Date Revised 20.12.2021
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Due to the public health concerns of endocrine-disrupting chemicals, there is an increasing demand to develop improved high-throughput detection assays for enhanced exposure control and risk assessment. A substrate-free, autobioluminescent HEK293ARE/Gal4-Lux assay was developed to screen compounds for their ability to induce androgen receptor (AR)-mediated transcriptional activation. The assay was validated against a group of 40 recommended chemicals and achieved an overall 87.5% accuracy in qualitatively classifying positive and negative AR agonists. The HEK293ARE/Gal4-Lux assay was demonstrated as a suitable tool for Tier 1 AR agonist screening. By eliminating exogenous substrate, this assay provided a significant advantage over traditional reporter assays by enabling higher-throughput screening with reduced testing costs while maintaining detection accuracy
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|i Enthalten in
|t BioTechniques
|d 1993
|g 71(2021), 2 vom: 05. Aug., Seite 403-415
|w (DE-627)NLM012627046
|x 1940-9818
|7 nnas
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| 773 |
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|g volume:71
|g year:2021
|g number:2
|g day:05
|g month:08
|g pages:403-415
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|u http://dx.doi.org/10.2144/btn-2021-0017
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