Rapid microfluidic isolation of virally infected primary bronchial epithelial cells for single-cell RNA sequencing
Single-cell RNA sequencing (scRNA-seq) of the bronchial epithelium enables examination of cellular subtypes and their responses to viral infections. Here, an optimized method for the isolation of virally infected primary bronchial epithelial cells using a commercially available microfluidic device i...
Ausführliche Beschreibung
Bibliographische Detailangaben
| Veröffentlicht in: | BioTechniques. - 1991. - 71(2021), 1 vom: 16. Juli, Seite 387-391
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| 1. Verfasser: |
Kimbley, Lucy M
(VerfasserIn) |
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| Weitere Verfasser: |
Parker, Rachel,
Jongen, Maaike Sybil,
Holloway, John W,
Swindle, Emily J,
Rose-Zerilli, Matthew Jj |
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| Format: | Online-Aufsatz
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| Sprache: | English |
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| Veröffentlicht: |
2021
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| Zugriff auf das übergeordnete Werk: | BioTechniques
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| Schlagworte: | Journal Article
bronchial epithelium
microfluidic device
scRNA-seq
single cell isolation
virus |
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