Rapid microfluidic isolation of virally infected primary bronchial epithelial cells for single-cell RNA sequencing
Single-cell RNA sequencing (scRNA-seq) of the bronchial epithelium enables examination of cellular subtypes and their responses to viral infections. Here, an optimized method for the isolation of virally infected primary bronchial epithelial cells using a commercially available microfluidic device i...
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Détails bibliographiques
| Publié dans: | BioTechniques. - 1991. - 71(2021), 1 vom: 16. Juli, Seite 387-391
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| Auteur principal: |
Kimbley, Lucy M
(Auteur) |
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| Autres auteurs: |
Parker, Rachel,
Jongen, Maaike Sybil,
Holloway, John W,
Swindle, Emily J,
Rose-Zerilli, Matthew Jj |
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| Format: | Article en ligne
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| Langue: | English |
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| Publié: |
2021
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| Accès à la collection: | BioTechniques
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| Sujets: | Journal Article
bronchial epithelium
microfluidic device
scRNA-seq
single cell isolation
virus |
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