Ladder-shape melting temperature isothermal amplification of nucleic acids
A novel method, termed ladder-shape melting temperature isothermal amplification (LMTIA), was developed in this study. As a proof of concept, one pair of primers or two pairs of nested primers and a thermostable DNA polymerase were employed to amplify the internal transcribed spacer of Oryza sativa...
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Bibliographische Detailangaben
| Veröffentlicht in: | BioTechniques. - 1993. - 71(2021), 1 vom: 27. Juli, Seite 358-369
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| 1. Verfasser: |
Wang, Deguo
(VerfasserIn) |
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| Weitere Verfasser: |
Wang, Yongzhen,
Zhang, Meng,
Zhang, Yongqing,
Sun, Juntao,
Song, Chunmei,
Xiao, Fugang,
Ping, Yuan,
Pan, Chen,
Hu, Yushan,
Wang, Chaoqun,
Liu, Yanhong |
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| Format: | Online-Aufsatz
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| Sprache: | English |
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| Veröffentlicht: |
2021
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| Zugriff auf das übergeordnete Werk: | BioTechniques
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| Schlagworte: | Journal Article
Research Support, Non-U.S. Gov't
isothermal amplification
ladder-shape melting temperature isothermal amplification (LMTIA)
loop-mediated isothermal amplification (LAMP)
melting curve
nucleic acids detection
primer design
DNA Primers
Nucleic Acids |
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