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231225s1994 xx |||||o 00| ||eng c |
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|a 10.1111/j.1469-8137.1994.tb04277.x
|2 doi
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|a pubmed24n1080.xml
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|a (DE-627)NLM324291078
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|a (NLM)33874514
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Bonnett, G D
|e verfasserin
|4 aut
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|a Purification and characterization of fructans with β-2, 1- and β-2, 6-glycosidic linkages suitable for enzyme studies
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|c 1994
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Revised 20.04.2021
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|a published: Print
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|a Citation Status PubMed-not-MEDLINE
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|a Fructan pentasaccharides were purified, in quantities suitable for use as substrates for enzyme assays, from Neosugar-p-(Meijj Seika Kaisha Ltd. Japan), tubers of Helianthus tuberosus L., L., and stems and leaf sheaths of Triticum aestivum L by a combination of gel-filtration and RP-HPLC. Fructan of higher molecular mass (mean DP = 30) was purified from Leaves of Lolium rigidum Gaud, that had been induced to accumulate fructan and characterized along; with the commercially available fructan from Cichorium intybus L. (Sigma, St Louis, USA) (mean DP = 33). The fructan pentasaccharide purified from H. tuberosus was found to contain exclusively 2, 1-linked fructose and terminal fructose and terminal glucose, and was identified as (1, 1, 1)-kestopentatise. The fructan pentasaccharide purified from Neosugar-P also contained (1,1,1)-kestopentaose. although the presence of fructan Klinked glucose and 1 % 2, 6-linked fructose indicated that a small proportion of other kestopentaoses were present, The fructan pentasaccharide purified from T aestivum consisted of almost exclusively 2,6-linked fructose and terminal glucose and terminal fructose and was considered to contain predominantly (6,6,6)-kestopentaose. The presence of 1 % 2,1,6)-linked fructose indicated the sample also contained a small proportion of branched kestopentanse. The high molecular mass fructan from C. intybus was found to comprise linear molecules containing only 2,1-linked fructose, terminal glucose and terminal fructose- High molecular mass fructan from L. rigidum contained predominantly 2. h-linked fructose, had predominantly internal glucose, indicated by 2 %, 1.6-linked glucose, low levels of branching, indicated 2 % 2,1,6-linked fructose residues; and 1% of the residues were 2,1 -linked fructose
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|a Journal Article
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|a (1,1,1)kestopentaose
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|a (6,6,6)-kestopentaose
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| 650 |
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|a Cichorium intybus
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| 650 |
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|a Fructan pentasaccharides
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| 650 |
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|a Lolium rigidum
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|a Sims, I M
|e verfasserin
|4 aut
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|a St John, J A
|e verfasserin
|4 aut
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|a Simpson, R J
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t The New phytologist
|d 1979
|g 127(1994), 2 vom: 20. Juni, Seite 261-269
|w (DE-627)NLM09818248X
|x 1469-8137
|7 nnns
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| 773 |
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|g volume:127
|g year:1994
|g number:2
|g day:20
|g month:06
|g pages:261-269
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|u http://dx.doi.org/10.1111/j.1469-8137.1994.tb04277.x
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|h 261-269
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