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231225s2002 xx |||||o 00| ||eng c |
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|a 10.1046/j.1469-8137.2002.00516.x
|2 doi
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|a pubmed24n1080.xml
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|a (NLM)33873274
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Kreuzwieser, Jürgen
|e verfasserin
|4 aut
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|a Xylem-transported glucose as an additional carbon source for leaf isoprene formation in Quercus robur
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|c 2002
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Revised 20.04.2021
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|a published: Print
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|a Citation Status PubMed-not-MEDLINE
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|a • In order to test whether xylem-transported carbohydrates are a potential source for isoprene biosynthesis, [U- 13 C]-labelled α- d -glucose was fed via cut ends of stems into the xylem of Quercus robur seedlings and the incorporation of 13 C into isoprene emitted was studied. Emission of 13 C-labelled isoprene was monitored in real time by proton-transfer-reaction mass spectrometry (PTR-MS). • A rapid incorporation of 13 C from xylem-fed glucose into single (mass 70) and double (mass 71) 13 C-labelled isoprene molecules was observed after a lag phase of approx. 5-10 min. This incorporation was temperature dependent and was highest (up to 13% 13 C of total carbon emitted as isoprene) at the temperature optimum of isoprene emission (40-42°C), when net assimilation was strongly reduced. • Fast dark-to-light transitions led to a strong single or double 13 C-labelling of isoprene from xylem-fed [U-13C]glucose. During a period of 10-15 min up to 86% of all isoprene molecules became single or double 13 C-labelled, resulting in a 13 C-portion of up to 27% of total carbon emitted as isoprene. • The results provide evidence that xylem-transported glucose or its degradation products can potentially be used as additional precursors for isoprene biosynthesis and that this carbon source becomes more important under conditions of limited photosynthesis
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|a Journal Article
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|a 13C-glucose
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|a Quercus robur
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|a alternative carbon source
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|a isoprene emission
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|a pedunculate oak
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|a proton-transfer-reaction mass spectrometry (PTR-MS)
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|a stable C-isotopes
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| 650 |
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4 |
|a xylem transport
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| 700 |
1 |
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|a Graus, Martin
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wisthaler, Armin
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Hansel, Armin
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Rennenberg, Heinz
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Schnitzler, Jörg-Peter
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t The New phytologist
|d 1979
|g 156(2002), 2 vom: 20. Nov., Seite 171-178
|w (DE-627)NLM09818248X
|x 1469-8137
|7 nnns
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| 773 |
1 |
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|g volume:156
|g year:2002
|g number:2
|g day:20
|g month:11
|g pages:171-178
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