Xylem-transported glucose as an additional carbon source for leaf isoprene formation in Quercus robur
• In order to test whether xylem-transported carbohydrates are a potential source for isoprene biosynthesis, [U- 13 C]-labelled α- d -glucose was fed via cut ends of stems into the xylem of Quercus robur seedlings and the incorporation of 13 C into isoprene emitted was studied. Emission of 13 C-lab...
| Veröffentlicht in: | The New phytologist. - 1979. - 156(2002), 2 vom: 20. Nov., Seite 171-178 |
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| Weitere Verfasser: | , , , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2002
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| Zugriff auf das übergeordnete Werk: | The New phytologist |
| Schlagworte: | Journal Article 13C-glucose Quercus robur alternative carbon source isoprene emission pedunculate oak proton-transfer-reaction mass spectrometry (PTR-MS) stable C-isotopes xylem transport |
| Zusammenfassung: | • In order to test whether xylem-transported carbohydrates are a potential source for isoprene biosynthesis, [U- 13 C]-labelled α- d -glucose was fed via cut ends of stems into the xylem of Quercus robur seedlings and the incorporation of 13 C into isoprene emitted was studied. Emission of 13 C-labelled isoprene was monitored in real time by proton-transfer-reaction mass spectrometry (PTR-MS). • A rapid incorporation of 13 C from xylem-fed glucose into single (mass 70) and double (mass 71) 13 C-labelled isoprene molecules was observed after a lag phase of approx. 5-10 min. This incorporation was temperature dependent and was highest (up to 13% 13 C of total carbon emitted as isoprene) at the temperature optimum of isoprene emission (40-42°C), when net assimilation was strongly reduced. • Fast dark-to-light transitions led to a strong single or double 13 C-labelling of isoprene from xylem-fed [U-13C]glucose. During a period of 10-15 min up to 86% of all isoprene molecules became single or double 13 C-labelled, resulting in a 13 C-portion of up to 27% of total carbon emitted as isoprene. • The results provide evidence that xylem-transported glucose or its degradation products can potentially be used as additional precursors for isoprene biosynthesis and that this carbon source becomes more important under conditions of limited photosynthesis |
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| Beschreibung: | Date Revised 20.04.2021 published: Print Citation Status PubMed-not-MEDLINE |
| ISSN: | 1469-8137 |
| DOI: | 10.1046/j.1469-8137.2002.00516.x |