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231225s2021 xx |||||o 00| ||eng c |
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|a 10.1111/vcp.12993
|2 doi
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|a pubmed25n1079.xml
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|a (DE-627)NLM324023677
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|a (NLM)33847384
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Moore, A Russell
|e verfasserin
|4 aut
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|a Diagnostic performance of routine electrophoresis and immunofixation for the detection of immunoglobulin paraproteins (M-Proteins) in dogs with multiple myeloma and related disorders
|b Part 1 - Current performance
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|c 2021
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 30.08.2021
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|a Date Revised 30.08.2021
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2021 American Society for Veterinary Clinical Pathology.
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|a BACKGROUND: Routine electrophoresis [agarose gel electrophoresis (AGE) and capillary zone electrophoresis (CZE)] and species-specific immunofixation (IF) can be used alone or in combination to detect immunoglobulin paraprotein (M-protein) and diagnose secretory myeloma-related disorders (sMRD)
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|a OBJECTIVE: We aimed to evaluate the performance of AGE, CZE, CZE plus IF (CZE-IF), and AGE plus IF (AGE-IF) for detecting canine serum M-proteins
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|a METHODS: One hundred canine cases that had AGE, CZE, and routine IF performed on serum, and where B-cell lineage neoplasia (such as B-cell lymphoma and plasma cell tumors) had been diagnosed or excluded, were evaluated. Routine IF protocols targeted IgG-FC, IgA, and IgM heavy chains and light chains. IgG4 IF and free light chain IF were also performed. B-cell lineage neoplasms with an M-protein detected, using any available method, were classified as sMRD. Datasets from AGE, CZE, IF, CZE-IF, and AGE-IF (electrophoretograms, gel images, and fraction concentrations) were composed and reviewed. The sensitivity, specificity, and Youden's index for M-protein detection were determined for each dataset
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|a RESULTS: The combination of AGE-IF or CZE-IF was more sensitive (82.9%) than CZE alone (72.0%) or AGE alone (64.6%) and more specific (66.1%, 48.3%, 51.7%, respectively). Immunofixation could be used alone to detect M-proteins (sensitivity 82.9%, specificity 61.9%), but there were technical challenges that complicated the performance and evaluation of the test. Myeloma with free light chains only was found in 5/41 cases of sMRD
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|a CONCLUSIONS: Adding routine IF to routine electrophoresis increases the ability to accurately identify M-proteins; however, there is still room for further diagnostic performance improvements
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|a Journal Article
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|a Canis lupis familiaris
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| 650 |
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|a Ehrlichia
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| 650 |
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|a biclonal gammopathy
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| 650 |
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|a canine
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| 650 |
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|a immunotyping
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| 650 |
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|a monoclonal gammopathy
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| 650 |
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|a Paraproteins
|2 NLM
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|a Harris, R Adam
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Jeffries, Christina
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Ashton, Laura
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Avery, Paul R
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Veterinary clinical pathology
|d 1975
|g 50(2021), 2 vom: 03. Juni, Seite 240-248
|w (DE-627)NLM098159984
|x 1939-165X
|7 nnas
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| 773 |
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|g volume:50
|g year:2021
|g number:2
|g day:03
|g month:06
|g pages:240-248
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|u http://dx.doi.org/10.1111/vcp.12993
|3 Volltext
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