Spatiotemporal patterns of intracellular Ca2+ signalling govern hypo-osmotic stress resilience in marine diatoms
© 2020 The Authors New Phytologist © 2020 New Phytologist Foundation.
| Veröffentlicht in: | The New phytologist. - 1979. - 230(2021), 1 vom: 30. Apr., Seite 155-170 |
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| 1. Verfasser: | |
| Weitere Verfasser: | , , , , , , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2021
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| Zugriff auf das übergeordnete Werk: | The New phytologist |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't Phaeodactylum Ca2+ signalling R-GECO1 algae diatoms environmental sensing osmotic stress signalling mehr... |
| Zusammenfassung: | © 2020 The Authors New Phytologist © 2020 New Phytologist Foundation. Diatoms are globally important phytoplankton that dominate coastal and polar-ice assemblages. These environments exhibit substantial changes in salinity over dynamic spatiotemporal regimes. Rapid sensory systems are vital to mitigate the harmful consequences of osmotic stress. Population-based analyses have suggested that Ca2+ signalling is involved in diatom osmotic sensing. However, mechanistic insight of the role of osmotic Ca2+ signalling is limited. Here, we show that Phaeodactylum Ca2+ elevations are essential for surviving hypo-osmotic shock. Moreover, employing novel single-cell imaging techniques we have characterised real-time Ca2+ signalling responses in single diatom cells to environmental osmotic perturbations. We observe that intracellular spatiotemporal patterns of osmotic-induced Ca2+ elevations encode vital information regarding the nature of the osmotic stimulus. Localised Ca2+ signals evoked by mild or gradual hypo-osmotic shocks are propagated globally from the apical cell tips, enabling fine-tuned cell volume regulation across the whole cell. Finally, we demonstrate that diatoms adopt Ca2+ -independent and dependent mechanisms for osmoregulation. We find that efflux of organic osmolytes occurs in a Ca2+ -independent manner, but this response is insufficient to mitigate cell damage during hypo-osmotic shock. By comparison, Ca2+ -dependent signalling is necessary to prevent cell bursting via precise coordination of K+ transport, and therefore is likely to underpin survival in dynamic osmotic environments |
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| Beschreibung: | Date Completed 14.05.2021 Date Revised 16.07.2022 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1469-8137 |
| DOI: | 10.1111/nph.17162 |