A simple electrochemiluminesecence aptasenor using a GCE/NCQDs/aptamers for detection of Pb

An electrochemiluminescence (ECL) aptasensor was prepared to detect Pb2+ with nitrogen-doped carbon quantum dots (NCQDs) as ECL materials. To prepare the working electrode, NCQDs with carboxyl groups were loaded on a glassy carbon electrode (GCE) and then Pb2+ aptamers were covalently bound to the N...

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Veröffentlicht in:Environmental technology. - 1993. - 43(2022), 15 vom: 01. Juni, Seite 2270-2277
1. Verfasser: Li, Danyang (VerfasserIn)
Weitere Verfasser: Chen, Chi, Guo, Xuefei, Liu, Changxia, Yang, Wensheng
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2022
Zugriff auf das übergeordnete Werk:Environmental technology
Schlagworte:Journal Article Nitrogen-doped carbon quantum dot Pb2+ aptamer aptasensor electrochemiluminescence Aptamers, Nucleotide Lead 2P299V784P Carbon mehr... 7440-44-0 Nitrogen N762921K75
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520 |a An electrochemiluminescence (ECL) aptasensor was prepared to detect Pb2+ with nitrogen-doped carbon quantum dots (NCQDs) as ECL materials. To prepare the working electrode, NCQDs with carboxyl groups were loaded on a glassy carbon electrode (GCE) and then Pb2+ aptamers were covalently bound to the NCQDs to form a stable GCE/NCQDs/aptamers. On addition of Pb2+, the chain aptamers change to a pb2+ G-quadruplex conformation, which lead to a large decrease in the ECL intensity. The variation of intensity and the logarithm of the Pb2+ concentration had a good linear relationship (R2 = 0.998). The detection range was wide (50 pM to 387.9 nM) with a low detection limit (18.9 pM). In interference experiments, the ECL Pb2+ aptasensor did not suffer from interference and it had good stability. The NCQDs ECL aptasensor can detect Pb2+ quickly and accurately, and provides a fast and efficient method for detection of Pb2+. Compared with literatures, the Pb2+ aptasensor has simpler preparation process, lower cost; furthermore, it is more environmentally friendly 
650 4 |a Journal Article 
650 4 |a Nitrogen-doped carbon quantum dot 
650 4 |a Pb2+ 
650 4 |a aptamer 
650 4 |a aptasensor 
650 4 |a electrochemiluminescence 
650 7 |a Aptamers, Nucleotide  |2 NLM 
650 7 |a Lead  |2 NLM 
650 7 |a 2P299V784P  |2 NLM 
650 7 |a Carbon  |2 NLM 
650 7 |a 7440-44-0  |2 NLM 
650 7 |a Nitrogen  |2 NLM 
650 7 |a N762921K75  |2 NLM 
700 1 |a Chen, Chi  |e verfasserin  |4 aut 
700 1 |a Guo, Xuefei  |e verfasserin  |4 aut 
700 1 |a Liu, Changxia  |e verfasserin  |4 aut 
700 1 |a Yang, Wensheng  |e verfasserin  |4 aut 
773 0 8 |i Enthalten in  |t Environmental technology  |d 1993  |g 43(2022), 15 vom: 01. Juni, Seite 2270-2277  |w (DE-627)NLM098202545  |x 1479-487X  |7 nnns 
773 1 8 |g volume:43  |g year:2022  |g number:15  |g day:01  |g month:06  |g pages:2270-2277 
856 4 0 |u http://dx.doi.org/10.1080/09593330.2021.1871661  |3 Volltext 
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