A simple electrochemiluminesecence aptasenor using a GCE/NCQDs/aptamers for detection of Pb
An electrochemiluminescence (ECL) aptasensor was prepared to detect Pb2+ with nitrogen-doped carbon quantum dots (NCQDs) as ECL materials. To prepare the working electrode, NCQDs with carboxyl groups were loaded on a glassy carbon electrode (GCE) and then Pb2+ aptamers were covalently bound to the N...
| Veröffentlicht in: | Environmental technology. - 1993. - 43(2022), 15 vom: 01. Juni, Seite 2270-2277 |
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| Weitere Verfasser: | , , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2022
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| Zugriff auf das übergeordnete Werk: | Environmental technology |
| Schlagworte: | Journal Article Nitrogen-doped carbon quantum dot Pb2+ aptamer aptasensor electrochemiluminescence Aptamers, Nucleotide Lead 2P299V784P Carbon mehr... |
| Zusammenfassung: | An electrochemiluminescence (ECL) aptasensor was prepared to detect Pb2+ with nitrogen-doped carbon quantum dots (NCQDs) as ECL materials. To prepare the working electrode, NCQDs with carboxyl groups were loaded on a glassy carbon electrode (GCE) and then Pb2+ aptamers were covalently bound to the NCQDs to form a stable GCE/NCQDs/aptamers. On addition of Pb2+, the chain aptamers change to a pb2+ G-quadruplex conformation, which lead to a large decrease in the ECL intensity. The variation of intensity and the logarithm of the Pb2+ concentration had a good linear relationship (R2 = 0.998). The detection range was wide (50 pM to 387.9 nM) with a low detection limit (18.9 pM). In interference experiments, the ECL Pb2+ aptasensor did not suffer from interference and it had good stability. The NCQDs ECL aptasensor can detect Pb2+ quickly and accurately, and provides a fast and efficient method for detection of Pb2+. Compared with literatures, the Pb2+ aptasensor has simpler preparation process, lower cost; furthermore, it is more environmentally friendly |
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| Beschreibung: | Date Completed 02.06.2022 Date Revised 02.06.2022 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1479-487X |
| DOI: | 10.1080/09593330.2021.1871661 |