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231225s2020 xx |||||o 00| ||eng c |
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|a 10.2166/wst.2020.525
|2 doi
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|a pubmed24n1063.xml
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|a DE-627
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|e rakwb
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|a eng
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1 |
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|a Nopprapun, Pimchanok
|e verfasserin
|4 aut
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|a Evaluation of a human-associated genetic marker for Escherichia coli (H8) for fecal source tracking in Thailand
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|c 2020
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 22.12.2020
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|a Date Revised 22.12.2020
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|a published: Print
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|a Citation Status MEDLINE
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|a High levels of microbial fecal pollution are a major concern in many countries. A human-associated genetic marker for Escherichia coli (H8) has recently been developed for fecal source tracking. The assessment of the H8 marker performance is crucial before it can be applied as a suitable method for fecal source tracking in each country. The performance (specificity and sensitivity) of the H8 marker was evaluated by using non-target host groups (cattle, buffalo, chicken, duck, and pig feces) and target host groups (influent and effluent from a wastewater treatment plant and septages). SYBR based real-time PCR (polymerase chain reaction) was done on 400 E. coli isolates from non-target and target host groups after E. coli isolation. It was found that the specificity from animal feces samples collected in Thailand was 96%. Moreover, influent, effluent, and septage samples showed the values of the sensitivity at 18, 12, and 36%, respectively. All of the non-target host groups were found to be significantly different with positive proportions from the target host group (septage samples) (p ≤ 0.01). Based on the results, this marker is recommended for use as a human-associated E. coli marker for identifying sources of fecal pollution in Thailand
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|a Journal Article
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|a Genetic Markers
|2 NLM
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1 |
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|a Boontanon, Suwanna Kitpati
|e verfasserin
|4 aut
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|a Harada, Hidenori
|e verfasserin
|4 aut
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1 |
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|a Surinkul, Nawatch
|e verfasserin
|4 aut
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|a Fujii, Shigeo
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Water science and technology : a journal of the International Association on Water Pollution Research
|d 1986
|g 82(2020), 12 vom: 31. Dez., Seite 2929-2936
|w (DE-627)NLM098149431
|x 0273-1223
|7 nnns
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| 773 |
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|g volume:82
|g year:2020
|g number:12
|g day:31
|g month:12
|g pages:2929-2936
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|u http://dx.doi.org/10.2166/wst.2020.525
|3 Volltext
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