Evaluation of a human-associated genetic marker for Escherichia coli (H8) for fecal source tracking in Thailand

High levels of microbial fecal pollution are a major concern in many countries. A human-associated genetic marker for Escherichia coli (H8) has recently been developed for fecal source tracking. The assessment of the H8 marker performance is crucial before it can be applied as a suitable method for...

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Veröffentlicht in:Water science and technology : a journal of the International Association on Water Pollution Research. - 1986. - 82(2020), 12 vom: 31. Dez., Seite 2929-2936
1. Verfasser: Nopprapun, Pimchanok (VerfasserIn)
Weitere Verfasser: Boontanon, Suwanna Kitpati, Harada, Hidenori, Surinkul, Nawatch, Fujii, Shigeo
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2020
Zugriff auf das übergeordnete Werk:Water science and technology : a journal of the International Association on Water Pollution Research
Schlagworte:Journal Article Genetic Markers
Beschreibung
Zusammenfassung:High levels of microbial fecal pollution are a major concern in many countries. A human-associated genetic marker for Escherichia coli (H8) has recently been developed for fecal source tracking. The assessment of the H8 marker performance is crucial before it can be applied as a suitable method for fecal source tracking in each country. The performance (specificity and sensitivity) of the H8 marker was evaluated by using non-target host groups (cattle, buffalo, chicken, duck, and pig feces) and target host groups (influent and effluent from a wastewater treatment plant and septages). SYBR based real-time PCR (polymerase chain reaction) was done on 400 E. coli isolates from non-target and target host groups after E. coli isolation. It was found that the specificity from animal feces samples collected in Thailand was 96%. Moreover, influent, effluent, and septage samples showed the values of the sensitivity at 18, 12, and 36%, respectively. All of the non-target host groups were found to be significantly different with positive proportions from the target host group (septage samples) (p ≤ 0.01). Based on the results, this marker is recommended for use as a human-associated E. coli marker for identifying sources of fecal pollution in Thailand
Beschreibung:Date Completed 22.12.2020
Date Revised 22.12.2020
published: Print
Citation Status MEDLINE
ISSN:0273-1223
DOI:10.2166/wst.2020.525