Transcriptome of airway neutrophils reveals an interferon response in life-threatening respiratory syncytial virus infection

Transcriptome of airway neutrophils reveals an interferon response in life-threatening respiratory syncytial virus infection

Copyright © 2020 The Author(s). Published by Elsevier Inc. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Clinical immunology (Orlando, Fla.). - 1999. - 220(2020) vom: 28. Nov., Seite 108593
1. Verfasser: Besteman, Sjanna B (VerfasserIn)
Weitere Verfasser: Callaghan, Amie, Langedijk, Annefleur C, Hennus, Marije P, Meyaard, Linde, Mokry, Michal, Bont, Louis J, Calis, Jorg J A
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2020
Zugriff auf das übergeordnete Werk:Clinical immunology (Orlando, Fla.)
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Bronchiolitis Immunity Interferon Neutrophil Respiratory syncytial virus Sputum Transcriptome B-Cell Activating Factor mehr... FCER1G, human NF-kappa B Receptors, Fc TNFSF13B protein, human RNA 63231-63-0 Interferons 9008-11-1
Beschreibung
Zusammenfassung:Copyright © 2020 The Author(s). Published by Elsevier Inc. All rights reserved.
BACKGROUND: Neutrophils are the most abundant cell type infiltrating the airways during severe respiratory syncytial virus (RSV) infection. Their exact role in disease pathophysiology remains enigmatic. Therefore, we determined genome-wide RNA expression profiles of local and systemic neutrophils in RSV bronchiolitis to provide further insight into local neutrophil biology
METHODS: We performed a single-center analysis, in 16 infants, admitted to the pediatric intensive care unit with severe RSV bronchiolitis. Neutrophils were isolated from blood and tracheobronchial aspirates (sputum). After low input RNA sequencing, differential expression of genes was determined followed by gene set analysis
RESULTS: Paired transcriptomic analysis of airway versus blood neutrophils showed an inflammatory phenotype, characterized by NF-kB signaling and upregulated expression of IL-6 and interferon pathways. We observed distinct expression of neutrophil activation genes (TNFSF13B, FCER1G)
DISCUSSION: Our data indicate that airway neutrophils regulate their function at the transcriptional level in response to viral infection. It also suggests that local interferon drives the neutrophil response of severe RSV bronchiolitis
Beschreibung:Date Completed 14.05.2021
Date Revised 14.05.2021
published: Print-Electronic
Citation Status MEDLINE
ISSN:1521-7035
DOI:10.1016/j.clim.2020.108593